Abstract
The bioassay-guided phytochemical investigation of Sarcococca hookeriana with respect to cholinesterase inhibitory properties has yielded two new 5alpha-pregnane-type steroidal alkaloids, hookerianamides J (1) and K (2), along with eight known compounds (3-10). The structures of 1 and 2 were elucidated by spectroscopic methods. These compounds displayed good to moderate activities in vitro against the enzymes acetylcholinesterase (IC 50 8.1-48.5 microM) and butyrylcholinesterase (IC 50 0.4-4.0 microM). Compounds 1-10 were also tested in vitro for their leishmanicidal activity against Leishmania major and for their antibacterial activities against Bacillus subtilis, Micrococcus luteus, Streptococcus faecalis, and Pseudomonas pallida.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Alkaloids / chemistry
-
Alkaloids / isolation & purification*
-
Alkaloids / pharmacology
-
Anti-Bacterial Agents / chemistry
-
Anti-Bacterial Agents / isolation & purification
-
Anti-Bacterial Agents / pharmacology
-
Antiprotozoal Agents / chemistry
-
Antiprotozoal Agents / isolation & purification
-
Antiprotozoal Agents / pharmacology
-
Buxaceae / chemistry*
-
Cholinesterase Inhibitors / chemistry
-
Cholinesterase Inhibitors / isolation & purification
-
Cholinesterase Inhibitors / pharmacology
-
Magnetic Resonance Spectroscopy
-
Microbial Sensitivity Tests
-
Molecular Structure
-
Pregnanes / chemistry*
-
Spectrometry, Mass, Electrospray Ionization
Substances
-
Alkaloids
-
Anti-Bacterial Agents
-
Antiprotozoal Agents
-
Cholinesterase Inhibitors
-
Pregnanes