Abstract
Amino-1,2,5-trideoxy-2,5-imino-D-mannitol was fluorescently tagged by reaction with dansyl chloride at N-1 or by attachment of a dansyl amide bearing spacer to this centre. Compounds obtained are highly potent inhibitors of beta-glucosidase exhibiting Ki values in the single figure nanomolar range. The 1-N-dansyl substituted inhibitor was successfully exploited for binding studies with beta-glucosidase from Agrobacterium sp. employing fluorescence spectrometric methods.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Binding, Competitive
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Dansyl Compounds / chemistry
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Dose-Response Relationship, Drug
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Electrophoresis, Polyacrylamide Gel / methods
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Enzyme Inhibitors* / chemical synthesis
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Enzyme Inhibitors* / metabolism
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Fluorescent Dyes / chemical synthesis
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Fluorescent Dyes / metabolism
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Glycoside Hydrolases / antagonists & inhibitors*
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Mannitol / chemical synthesis
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Mannitol / metabolism
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Molecular Probes / chemical synthesis
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Molecular Probes / metabolism
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Protein Binding
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Rhizobium / enzymology
Substances
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Dansyl Compounds
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Enzyme Inhibitors
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Fluorescent Dyes
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Molecular Probes
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Mannitol
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Glycoside Hydrolases
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dansyl chloride