Activity Spreadsheet -- Enzyme Inhibition Constant Data from BindingDB (change energy unit to kcal/mol)

Found 7 hits in this display   

TargetLysosomal acid glucosylceramidase(Homo sapiens (Human))
Nankai University

LigandPNGBDBM108217(2-(3,3-dimethylbutyl)-5-(hydroxymethyl)- 5H,6H,7H,...)
Affinity DataKi:  5.34nMpH: 5.2Assay Description:To determine inhibition constant (Ki), substrate (7.5µL, various concentrations in Mcllvaine buffer, pH 5.2) and enzyme (12.5µL, 0.1mg/mL) ...More data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetLysosomal acid glucosylceramidase(Homo sapiens (Human))
Nankai University

LigandPNGBDBM108217(2-(3,3-dimethylbutyl)-5-(hydroxymethyl)- 5H,6H,7H,...)
Affinity DataIC50:  4.26nMpH: 7.0Assay Description:The assays were performed at 37°C with 4-MU-β-ᴅ-Glu as the substrate in Mcllvaine buffer (sodium citrate (100mM) and sodium phosphate...More data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetLysosomal acid glucosylceramidase(Homo sapiens (Human))
Nankai University

LigandPNGBDBM108217(2-(3,3-dimethylbutyl)-5-(hydroxymethyl)- 5H,6H,7H,...)
Affinity DataIC50:  5.34nMpH: 5.2Assay Description:The assays were performed at 37°C with 4-MU-β-ᴅ-Glu as the substrate in Mcllvaine buffer (sodium citrate (100mM) and sodium phosphate...More data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetPrunasin hydrolase(Prunus dulcis (Almond))
Nankai University

LigandPNGBDBM108217(2-(3,3-dimethylbutyl)-5-(hydroxymethyl)- 5H,6H,7H,...)
Affinity DataIC50:  79nMpH: 10.6 T: 2°CAssay Description:In a 96-well plates, 10µL of commercial enzyme solutions without (control) or with 20µL of inhibitor were incubated at 37°C for 5 min....More data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetBeta-galactosidase(Bos taurus (Bovine))
Nankai University

LigandPNGBDBM108217(2-(3,3-dimethylbutyl)-5-(hydroxymethyl)- 5H,6H,7H,...)
Affinity DataIC50:  350nMpH: 10.6 T: 2°CAssay Description:In a 96-well plates, 10µL of commercial enzyme solutions without (control) or with 20µL of inhibitor were incubated at 37°C for 5 min....More data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetOligo-1,6-glucosidase IMA1(Saccharomyces cerevisiae S288c (Baker's yeast))
Nankai University

LigandPNGBDBM108217(2-(3,3-dimethylbutyl)-5-(hydroxymethyl)- 5H,6H,7H,...)
Affinity DataIC50:  3.42E+3nMpH: 10.6 T: 2°CAssay Description:In a 96-well plates, 10µL of commercial enzyme solutions without (control) or with 20µL of inhibitor were incubated at 37°C for 5 min....More data for this Ligand-Target Pair
In DepthDetails ArticlePubMed
TargetAlpha-galactosidase(Coffea arabica (Coffee beans))
Nankai University

LigandPNGBDBM108217(2-(3,3-dimethylbutyl)-5-(hydroxymethyl)- 5H,6H,7H,...)
Affinity DataIC50:  1.28E+4nMpH: 10.6 T: 2°CAssay Description:In a 96-well plates, 10µL of commercial enzyme solutions without (control) or with 20µL of inhibitor were incubated at 37°C for 5 min....More data for this Ligand-Target Pair
In DepthDetails ArticlePubMed