Affinity DataKi: 0.100nMAssay Description:Binding affinity to NAALADaseMore data for this Ligand-Target Pair
3D Structure (crystal)
Affinity DataKi: 0.200nMAssay Description:In vitro inhibitory activity against glutamate carboxypeptidase II (GCP II) using N-acetyl-L-aspartyl-[3H]-L-glutamate as a substrateMore data for this Ligand-Target Pair
Affinity DataKi: 0.200nMAssay Description:Concentration of the compound required for the neuroprotective effect determined by inhibition of GCP IIMore data for this Ligand-Target Pair
Affinity DataKi: 0.275nMAssay Description:Compound was tested for inhibition of the Folate hydrolaseMore data for this Ligand-Target Pair
Affinity DataKi: 0.280nMAssay Description:Inhibition of antiTEV-tagged GCP2 extracellular portion (aa 44-750) (unknown origin) using folyl-di-L-glutamate as substrate by HPLC-based enzymatic ...More data for this Ligand-Target Pair
Affinity DataKi: 0.300nMAssay Description:Inhibition of GCPII (unknown origin) NAALADase activityMore data for this Ligand-Target Pair
Affinity DataKi: 0.300nMAssay Description:Binding affinity to NAALADaseMore data for this Ligand-Target Pair
Affinity DataKi: 0.400nMAssay Description:Inhibition of NAALADase activity in human LNCaP cell membranes assessed as inhibition of [3H]NAG conversion to [3H]glutamate after 30 mins by liquid ...More data for this Ligand-Target Pair
Affinity DataKi: 6.60nMAssay Description:The inhibitory activity of all compounds was determined using a fluorescent assay of human PSMA activity. The enzyme used in the assay was purchased ...More data for this Ligand-Target Pair
Affinity DataKi: 31nMAssay Description:Displacement of [123I]-DCIT from PSMA in human LNCaP cells after 1 hr by gamma countingMore data for this Ligand-Target Pair
Affinity DataIC50: 0.100nMpH: 7.4 T: 37°CAssay Description:GCPII activity in vitro is monitored through the hydrolysis [3H]NAAG to NAA and [3H]Glu. The radioactivity-based assay was miniaturized to a 96-well ...More data for this Ligand-Target Pair
Affinity DataIC50: 0.300nMAssay Description:Inhibition of human recombinant NAALADaseMore data for this Ligand-Target Pair
Affinity DataIC50: 0.300nMAssay Description:Inhibition of GCP-2 (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 0.300nMAssay Description:Inhibition of N-acetyl-L-aspartyl-[3H]-L-glutamate binding to glutamate carboxypeptidase II (GCP II)More data for this Ligand-Target Pair
Affinity DataIC50: 0.300nMAssay Description:Inhibitory concentration against rat brain NAALADase (Folate hydrolase)More data for this Ligand-Target Pair
Affinity DataIC50: 0.300nMAssay Description:Inhibition of Glutamate carboxypeptidase IIMore data for this Ligand-Target Pair
Affinity DataIC50: 0.300nMpH: 7.4 T: 37°CAssay Description:GCPII activity in vitro is monitored through the hydrolysis [3H]NAAG to NAA and [3H]Glu. The radioactivity-based assay was miniaturized to a 96-well ...More data for this Ligand-Target Pair
Affinity DataIC50: 0.300nMpH: 7.4 T: 37°CAssay Description:The inhibition concentration values of GCPII were determined using the radioenzymatic assay with [3H] NAAG (radiolabeled on the terminal glutamate). ...More data for this Ligand-Target Pair
Affinity DataIC50: 0.300nMpH: 7.4 T: 37°CAssay Description:GCPII activity in vitro is monitored through the hydrolysis [3H]NAAG to NAA and [3H]Glu. The radioactivity-based assay was miniaturized to a 96-well ...More data for this Ligand-Target Pair
Affinity DataIC50: 1.10nMAssay Description:Inhibition of NAALADase activity in human LNCaP cell membranes assessed as inhibition of [3H]NAG conversion to [3H]glutamate after 30 mins by liquid ...More data for this Ligand-Target Pair
Affinity DataIC50: 5.10nMAssay Description:Inhibitory activity of the compound against expressed rat Glutamate carboxypeptidase II, using a substrate concentration of 5 microMMore data for this Ligand-Target Pair
Affinity DataIC50: 52nMAssay Description:Displacement of [125I]23 from PSMA in human LNCAP cellsMore data for this Ligand-Target Pair
Affinity DataIC50: 80nMAssay Description:Inhibition of PSMA derived from human LNCaP cell lysate assessed as inhibition of NAAG hydrolysis incubated for 2 hrs in presence of NAAG by Amplex R...More data for this Ligand-Target Pair
Affinity DataIC50: 80nMAssay Description:Inhibition of PSMA in human LNCaP cell extracts using NAAG as substrate by amplex red reagent based assayMore data for this Ligand-Target Pair
Affinity DataIC50: 80nMAssay Description:Inhibition of GCPII (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 94nMAssay Description:Inhibition of GCPII (unknown origin) expressed in DU-145 cells after 2 hrs by Amplex red reagent based fluorescence assayMore data for this Ligand-Target Pair
Affinity DataKd: 0.400nMAssay Description:Binding affinity to PSMA in human LNCaP cells assessed as cell surface binding in Tris-buffered saline bufferMore data for this Ligand-Target Pair
Affinity DataEC50: 0.620nMAssay Description:Concentration of the compound required for the neuroprotective effect determined by inhibition of GCP IIMore data for this Ligand-Target Pair
Affinity DataKd: 0.5nMAssay Description:Binding affinity to PSMA in human PC3 cells assessed as cell surface binding in Tris-buffered saline bufferMore data for this Ligand-Target Pair
