Affinity DataIC50: 3.70nM Kd: 16nMpH: 7.4 T: 25°CAssay Description:The formation of ADP from ATP was quantified using a coupled enzyme assay (DiscoverX) in with a fluorescent resorufin dye is generated from the inter...More data for this Ligand-Target Pair
3D Structure (crystal)Affinity DataIC50: 3.70nMAssay Description:In vitro enzyme activity assay using Aurora Kinase A. More data for this Ligand-Target Pair
Affinity DataIC50: 3.70nMpH: 7.4 T: 25°CAssay Description:Reactions were carried out at room temperature in 15 mM HEPES buffer (pH 7.4) containing 20 mM NaCl, 1 mM EGTA, 0.02% Tween 20, 10 mM MgCl2, 5% (v/v)...More data for this Ligand-Target Pair
Affinity DataIC50: 3.70nMpH: 7.4 T: 18°CAssay Description:Aurora A was exchanged into 50 mM phosphate buffer (pH 7.4) including 1 mM DTT via PD-10 columns and was concentrated to 20 mg mL-1 using Amicon Ultr...More data for this Ligand-Target Pair
Affinity DataIC50: 3.70nM Kd: 16nMpH: 7.4Assay Description:The binding of inhibitors to Aurora A kinase was analyzed with a MicroCal iTC200 titration calorimeter (GE Healthcare, Piscataway, N.J.). The protein...More data for this Ligand-Target Pair
Affinity DataIC50: 3.70nMAssay Description:Inhibition of His6 tagged MBP fused human aurora A (123 to 390 residues) expressed in Escherichia coli Tuner (DE3) cellsMore data for this Ligand-Target Pair
Affinity DataKd: 16nMAssay Description:Binding affinity to His6 tagged MBP fused human aurora A (123 to 390 residues) expressed in Escherichia coli Tuner (DE3) cells by isothermal titratio...More data for this Ligand-Target Pair
