Found 59 Enz. Inhib. hit(s) for PDB: 1X70 
Affinity DataIC50: 3.5nMAssay Description:Inhibition of DPP4 in human plasma assessed as formation of 7-amino-4-methylcoumarin from glycyl-L-proline 4-methylcoumaryl-7-amide by fluorescence a...More data for this Ligand-Target Pair
3D Structure (crystal)Affinity DataIC50: 6nMAssay Description:Inhibition of human DPP4 expressed in HEK293 cells using Ala-Pro-p-nitroanilide hydrochloride salt as substrate preincubated for 10 mins prior to add...More data for this Ligand-Target Pair
Affinity DataIC50: 6.20nMAssay Description:Inhibition of DPP4 (unknown origin) expressed in Sf9 cells using Gly-Pro-AMC substrateMore data for this Ligand-Target Pair
Affinity DataIC50: 7.80nMAssay Description:Inhibition of recombinant human DPP4 using Gly-Pro-AMC as substrate after 1 hr by fluorescence assayMore data for this Ligand-Target Pair
Affinity DataIC50: 11nMT: 2°CAssay Description:A 200-µL reaction system containing DPP-IV (Sigma), a test compound, and 25 mmol/L HEPES buffer (containing 140 mmol/L NaCl, 1% BSA, and 80 mmol/L Mg...More data for this Ligand-Target Pair
Affinity DataIC50: 14nMAssay Description:Screening Method:Name of method: Activity Evaluation of DPP4, Fluorescence.Instrument: Microplate reader, Envision (PerkinElmer, USA).Material: human...More data for this Ligand-Target Pair
Affinity DataIC50: 16nMAssay Description:Inhibition of DPP4 (unknown origin) pre-incubated for 1 hr before Gly-Pro-pNA substrate addition and measured 2 hrs post substrate additionMore data for this Ligand-Target Pair
Affinity DataIC50: 17nMAssay Description:Inhibition of DPP4 (unknown origin) using Gly-Pro-p-nitroanilide as substrate incubated for 1 hrMore data for this Ligand-Target Pair
Affinity DataIC50: 18nMAssay Description:Inhibition of DPP4 in presence of 50% human serumMore data for this Ligand-Target Pair
Affinity DataIC50: 18nMAssay Description:Inhibition of human DPP-4 expressed in baculovirus expression system using H-Gly-Pro-AMC as substrate after 30 mins by fluorometric analysisMore data for this Ligand-Target Pair
Affinity DataIC50: 18nMAssay Description:Competitive reversible inhibition of DPP4 (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 18nMAssay Description:DPP-IV inhibitory activity was determined by measuring the p-nitroaniline (pNA) released from the chromogenic substrate hydrolysis (H-Gly-Pro-pNA). T...More data for this Ligand-Target Pair
Affinity DataIC50: 18nMAssay Description:The HypoGen module in DS2.5 was employed to produce pharmaphores with the training set compounds.More data for this Ligand-Target Pair
Affinity DataIC50: 18nMpH: 7.5 T: 2°CAssay Description:The enzyme activity resulted in the formation of the fluorescent product amidomethylcoumarin (AMC), which was monitored by excitation at 360 nm and m...More data for this Ligand-Target Pair
Affinity DataIC50: 18nMpH: 7.5 T: 2°CAssay Description:The enzyme activity resulted in the formation of the fluorescent product amidomethylcoumarin (AMC), which was monitored by excitation at 360 nm and m...More data for this Ligand-Target Pair
Affinity DataIC50: 18nMAssay Description:Inhibition of DPP4 in human plasma using Gly-Pro-AMC as substrate by fluorimetric analysisMore data for this Ligand-Target Pair
Affinity DataIC50: 18nMAssay Description:Inhibition of DPP-4 (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 18nMAssay Description:Inhibition of DPP4 (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 18nMAssay Description:Inhibition of human recombinant DPP-4 using Gly-pro-p-nitroanilide as substrate assessed as formation of p-nitroaniline after 30 mins by colorimetric...More data for this Ligand-Target Pair
Affinity DataIC50: 18nMAssay Description:Inhibition of human DPP4 expressed in baculovirus systemMore data for this Ligand-Target Pair
Affinity DataIC50: 18nMAssay Description:Inhibition of DPP4 activityMore data for this Ligand-Target Pair
Affinity DataIC50: 18nMAssay Description:Inhibition of human DPP-4 using H-Gly-Pro-para-Nitroanilide as substrate incubated for 20 mins prior to substrate addition measured after 30 mins by ...More data for this Ligand-Target Pair
Affinity DataIC50: 19nMAssay Description:Inhibition of DPP4 extracted from human Caco2 cells using H-Gly-Pro-AMC substrate after 10 mins by fluorescence assayMore data for this Ligand-Target Pair
Affinity DataIC50: 19nMAssay Description:Inhibition of DPP4 (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 19.4nMAssay Description:Inhibition of human DPP4 assessed as hydrolytic reaction of Ala-Pro-AMCMore data for this Ligand-Target Pair
Affinity DataIC50: 20nMAssay Description:Inhibition of human plasma DPP4More data for this Ligand-Target Pair
Affinity DataIC50: 20nMAssay Description:Inhibition of human recombinant his6-tagged DPP4 using Ala-Pro-AMC as substrate by continuous fluorometric methodMore data for this Ligand-Target Pair
Affinity DataIC50: 20nMAssay Description:Inhibition of human recombinant DPP4 expressed in baculovirus expression system using Ala-Pro-AMC as substrate by continuous fluorometric assayMore data for this Ligand-Target Pair
Affinity DataIC50: 20nMAssay Description:Inhibition of human recombinant DPP4 after 60 mins by fluorescence plate readerMore data for this Ligand-Target Pair
Affinity DataIC50: 22nMAssay Description:Inhibition of recombinant human DPP-4 expressed in HEK293 cells using H-Gly-Pro-AMC as substrate measured over 15 mins by fluorescence analysisMore data for this Ligand-Target Pair
Affinity DataIC50: 22nMAssay Description:Inhibition of human recombinant DPP4 using H-Gly-Pro-AMC substrate incubated for 15 mins by fluorescent AMC release assayMore data for this Ligand-Target Pair
Affinity DataIC50: 22nMpH: 7.5Assay Description:The reaction contained 10 uM of Ala-Pro-AMC, 50 pM of enzyme DDP-4, different concentrations of test compounds, and assay buffer (100 mM HEPES, pH 7....More data for this Ligand-Target Pair
Affinity DataIC50: 22nMAssay Description:Inhibition of recombinant human DPP-4 expressed in HEK293 cells using H-Gly-Pro-AMC as substrate measured over 15 mins by fluorescence analysisMore data for this Ligand-Target Pair
Affinity DataIC50: 23nMAssay Description:Inhibition of human recombinant His-tagged DPP4 expressed in insect cellsMore data for this Ligand-Target Pair
TargetDipeptidyl peptidase 4(Rattus norvegicus (rat))
Shanghai Sun-Sail Pharmaceutical Science & Technology
US Patent
Shanghai Sun-Sail Pharmaceutical Science & Technology
US Patent
Affinity DataIC50: 26.0nMAssay Description:Thoroughly mixed test compounds, enzyme and reaction buffer, preincubated the mixture for 15 minutes at 37° C. and then primed reaction by adding sub...More data for this Ligand-Target Pair
Affinity DataIC50: 29nMAssay Description:Inhibition of human recombinant DPP4 pre-incubated with compound for 15 mins before substrate addition by luminescence assayMore data for this Ligand-Target Pair
Affinity DataIC50: 30nMpH: 8.0 T: 2°CAssay Description:The enzyme activity resulted in the liberation of free pNA at 405 nm. Reaction progress was monitored using a Molecular Devices SpectraMax Plus micro...More data for this Ligand-Target Pair
Affinity DataIC50: 30nMAssay Description:Inhibition of DPP4 (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 30nMAssay Description:Inhibition of human DPP4More data for this Ligand-Target Pair
TargetDipeptidyl peptidase 4(Rattus norvegicus (rat))
Shanghai Sun-Sail Pharmaceutical Science & Technology
US Patent
Shanghai Sun-Sail Pharmaceutical Science & Technology
US Patent
Affinity DataIC50: 33nMAssay Description:Compound was tested in vitro for binding affinity against human carbonic anhydrase II; (ki*10e-9)More data for this Ligand-Target Pair
Affinity DataIC50: 38nMAssay Description:Inhibition of human recombinant C-terminal 6His-tagged DPP-4 expressed in insect cells incubated for 30 mins by TPE-KFPE fluorescent probe-based assa...More data for this Ligand-Target Pair
Affinity DataIC50: 40nMAssay Description:Inhibition of DPP4 (unknown origin)More data for this Ligand-Target Pair
Affinity DataIC50: 40nMAssay Description:Inhibition of human seminal plasma DPP4 assessed as pNA release from Gly-Pro-p-nitroanilide substrate pre-incubated with enzyme for 15 min prior to s...More data for this Ligand-Target Pair
