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Solution Informationhelp
Enzyme: LANA
inhibitor: BDBM74293
substrate: n/a
 
Solution Type: Aqueous
pH at Preparation: n/a
Temp. Prep.: n/a
Comments: LANA 1-23 peptide containing the first 23 amino acids of the LANA protein from Kaposi's sarcoma herpesvirus (KSHV) was synthesized with an N-terminal FITC via a beta alanine linker and HPLC purified. Purified chicken Erythrocyte nucleosomes are provided by the assay provider and purified from chicken red blood cells. They are stored at -80C at 30 uM. Assay buffer (TEN-BT) is 10 mM Tris-HCl (pH 7.5), 1 mM EDTA (pH 8.0), 2.5 mM NaCl, 5 mM Beta-mercaptoethanol, 0.01% Triton-X-100 LANA peptide is diluted to a final concentration of 50 nM in TEN-BT buffer with 100 nM of purified nucleosomes in black 1536 well plates with 10 uL per well. 7.5 nL of compounds are pinned per well. The reaction is incubated at room temperature for 60 minutes (but is stable for up to 2 hours). The assay is read on a Viewlux plate reader using a 480 nM excitation filter, 535nM S and P emission filters and D505fp/D535 dichoric mirror. mP value for FP measurement = 1000*(S-G*P)/(S+G*P) where S=
 
 

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Last update November 1, 2007
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