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Solution Informationhelp
Enzyme: Sphingosine 1-phosphate receptor 3
inhibitor: BDBM31058
substrate: n/a
 
Solution Type: Aqueous
pH at Preparation: n/a
Temp. Prep.: n/a
Comments: Assay Overview: Compounds identified from a previously described set of experiments entitled "Primary HTS assay for 5-Hydroxytryptamine (Serotonin) Receptor Subtype 1a (5HT1a) agonists" and "Primary Cell Based High Throughput Screening Assay for Agonists of the 5-Hydroxytryptamine Receptor Subtype 1E (5HT1E)" were selected for testing in this assay. Further information can be found by searching on this website for PubChem AID = 612 and Pubchem AID=571. The dose response curves for these compounds on the 5HT1E and 5HT1A receptors are in PubChem AID = 749 and Pubchem AID=755. A cell line containing the S1P3 receptor, the promiscuous G-alpha-15 protein (Ga15) as well as the beta-lactamase (BLA) reporter-gene under control of the nuclear factor of activated T-cells (NFAT) promoter was used to measure nonspecific compound effects. When the cells are stimulated transcription of the NFAT-BLA gene occurs via a Ga15 protein coupled signaling cascade. The amount of BLA activity is proportion
 
 

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Last update November 1, 2007
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