Reaction Details  Report a problem with these data
Cell Reactant:
Thymidine Kinase
Syringe Reactant:
BDBM1
Meas. Tech.:
ITC
Entry Date.:
12/13/00
ΔG°:
-7.13±0.35 (kcal/mole)
pH:
7.5±n/a
Temperature:
283.15±n/a (K)
ΔH° :
-13.67±0.68 (kJ/mole)
Corrected for ΔHioniz:
yes
Protons Released:
0
ΔCp :
-0.36±0.02 (kJ/mole)
ΔS° :
-0.02±0.01 (kJ/mole-K)
Citation
 Perozzo, R; Jelesarov, I; Bosshard, HR; Folkers, G; Scapozza, L Compulsory order of substrate binding to herpes simplex virus type 1 thymidine kinase. A calorimetric study. J Biol Chem 275:16139-45 (2000) [PubMed]  Article
Cell React
Source:
Expressed as glutathione S-transferase fusion protein in E. coli.
Prep. Method:
Purified by glutathione affinity chromatography.
Name:
Thymidine kinase
Synonyms:
KITH_HHV11 | TK
Type:
Enzyme
Mol. Mass.:
40978.57
Organism:
Human herpesvirus 1
Description:
P03176
Residue:
376
Sequence:
MASYPCHQHASAFDQAARSRGHNNRRTALRPRRQQEATEVRPEQKMPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRVLGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAVPPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFILDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN
  
Syringe React
Source:
Sigma
Name:
BDBM1
Synonyms:
dT | thymidine
Type:
Nucleoside or nucleotide
Emp. Form.:
C10H14N2O5
Mol. Mass.:
242.2286
SMILES:
Cc1cn([C@H]2C[C@H](O)[C@@H](CO)O2)c(=O)[nH]c1=O
Structure:
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