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Cell Reactant:HyHEL-5 Mutant(E50Q)
Syringe Reactant:Lysozyme (bobwhite quail)
Meas. Tech.:ITC and sedimentation equilibrium
Entry Date:12/13/00
 
ΔG°:-6.73±1.21 (kcal/mole)
pH:8±n/a
Log10Kb:5.08± n/a
Temperature:298.15±n/a (K)
ΔH° :n/a
ΔHobs:n/a
Ionic Strength:n/a
Corrected for ΔHioniz:not known
Protons Released:n/a
ΔCp :n/a
Stoich. Param.:n/a
ΔS° :n/a
Comments:affinity was too low to measured through ITC
 
Citation Wibbenmeyer, JASchuck, PSmith-Gill, SJWillson, RC Salt links dominate affinity of antibody HyHEL-5 for lysozyme through enthalpic contributions. J Biol Chem274:26838-42 (1999) [PubMed]  Article
More Info.:  Get all data from this article ,  ITC RUN data ,  Solution Info ,  Data Fit Method ,  Instrument Info
 
HyHEL-5 Mutant(E50Q)
Source:Site-directed mutagenesis of recombinant HyHEL-5 Fab
Purity:95%
Prep. Method:Refolded protein was purified by Q Sepharose and nickel-charged chelating Sepharose Fast Flow Chromatography
Name:HyHEL-5 Mutant(E50Q)
Synonyms:HyHEL-5 Fab mutant (E50Q)
Type:Antibody
Mol. Mass.:23011.87
Organism:Mus musculus (mouse)
Description:mutant of murine monoclonal antibody HyHEL-5 to hen egg lysozyme
Residue:215
Sequence:
EVQLQQSGAELMKPGASVKISCKASGYTFSDYWIEWVKQRPGHGLEWIGQILPGSGSTNY
HERFKGKATFTADTSSSTAYMQLNSLTSEDSGVYYCLHGNYDFDGWGQGTTLTVSSAKTT
PPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTL
SSSVTVPSSTWPSETVTCNVAHPASSTKVDKKILD
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Lysozyme (bobwhite quail)
Source:Prepared from eggs purchased from Stevenson's Game Bird Farm (Riverside, TX)
Purity:n/a
Prep. Method:n/a
Name:Lysozyme (bobwhite quail)
Synonyms:bobwhite quail lysozyme(BQL)
Type:Enzyme
Mol. Mass.:14279.53
Organism:Colinus virginianus
Description:hydrolase
Residue:129
Sequence:
KVFGRCELAAAMKRHGLDNYRGYSLGNWVCAAKFESNFNSQATNRNTDGSTDYGVLQINS
RWWCNDGKTPGSRNLCNIPCSALLSSDITATVNCAKKIVSDGNGMNAWVAWRNRCKGTDV
QAWIRGCRL
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