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Cell Reactant:Glyoxylate Regulatory Protein (C-AllR)
Syringe Reactant:BDBM19472
Meas. Tech.:Isothermal Titration Calorimetry
Entry Date:12/10/07
 
ΔG°:-4.43± (kJ/mole)
pH:7.4±n/a
Log10Kb:5.04± 4.48
Temperature:298.15±n/a (K)
ΔH° :n/a
ΔHobs :-34.3± (kJ/mole)
Ionic Strength:n/a
Corrected for ΔHioniz:no
Protons Released:n/a
ΔCp :n/a
Stoich. Param.:0.5
ΔS° : -0.1011± (kJ/mole-K)
Comments:n/a
 
Citation Lorca, GLEzersky, ALunin, VVWalker, JRAltamentova, SEvdokimova, EVedadi, MBochkarev, ASavchenko, A Glyoxylate and pyruvate are antagonistic effectors of the Escherichia coli IclR transcriptional regulator. J Biol Chem282:16476-91 (2007) [PubMed]  Article
More Info.:  Get all data from this article ,  ITC RUN data ,  Solution Info ,  Data Fit Method ,  Instrument Info
 
Glyoxylate Regulatory Protein (C-AllR)
Source:His-tagged fusion protein was overexpressed and purified in E. coli.
Purity:n/a
Prep. Method:The His6 tag was cleaved from the protein by tobacco etch virus protease. The cleaved protein was then resolved by flowing the mixture through a second Ni2+ column. The purified proteins were dialyzed and concentrated.
Name:Glyoxylate Regulatory Protein (C-AllR)
Synonyms:Negative regulator of allantoin and glyoxylate utilization operons | Transcriptional regulator AllR, C-terminal domain
Type:C-terminal effector-binding domain
Mol. Mass.:29267.82
Organism:Escherichia coli
Description:P0ACN4
Residue:271
Sequence:
MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVSDISLNLDLPLSTTFRL
LKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVAGPFMRRLMLLSGETVNVA
IRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKALLYPLAEEELMSIILQTGLQQ
FTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCIASAIYDDVGSVVAAISISGPSS
RLTEDRFVSQGELVRDTARDISTALGLKAHP
Blast this sequence in BindingDB or PDB
  Blast E-value cutoff:
BDBM19472
Source:n/a
Purity:n/a
Prep. Method:n/a
NameBDBM19472
Synonyms:2-oxoacetic acid | Glyoxylate | Oxoethanoic acid | glyoxylic acid | oxaldehydic acid
TypeSmall organic molecule
Emp. Form.C2H2O3
Mol. Mass.74.0355
SMILESOC(=O)C=O
Structure

   
    

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Last update November 1, 2007
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