Reaction Details 
Report a problem with these data
Report a problem with these dataCell Reactant:
Caspase-3
Syringe Reactant:
BDBM19728
Meas. Tech.:
Isothermal Titration Calorimetry
Entry Date.:
12/19/07
ΔG°:
-40.4206±n/a (kJ/mole)
pH:
7.2±n/a
Log10Kb:
6.4
Temperature:
298.15±n/a (K)
ΔHobs :
-10.2828±0.1254 (kJ/mole)
Corrected for ΔHioniz:
no
Stoich. Param.:
1.14
ΔS° :
0.1011±n/a (kJ/mole-K)
Citation
Aulabaugh, A; Kapoor, B; Huang, X; Dollings, P; Hum, WT; Banker, A; Wood, A; Ellestad, G Biochemical and biophysical characterization of inhibitor binding to caspase-3 reveals induced asymmetry. Biochemistry 46:9462-71 (2007) [PubMed] Article Cell React
Source:
The DNA sequence encoding the caspase-3 catalytic domain with a C-terminal hexa-His tag was cloned and expressed in Escherichia coli BL21 (DE3) cells.
Purity:
>95%
Prep. Method:
The cell extracts were further purified by Ni-NTA agarose and gel-filtration columns. The purified caspase-3 showed 2 subunits of 20 and 10 kDa on reduced SDS-PAGE.
Name:
Caspase-3
Synonyms:
Apopain | CASP-3 | CASP3 | CASP3_HUMAN | CPP-32 | CPP32 | Caspase 3 | Caspase-3 subunit p12 | Caspase-3 subunit p17 | Cysteine protease CPP32 | SCA-1 | SREBP cleavage activity 1 | Yama protein | caspase-3 preproprotein
Type:
Hydrolase; heterotetramer of two heterodimers
Mol. Mass.:
31607.55
Organism:
Homo sapiens (Human)
Description:
n/a
Residue:
277
Sequence:
MENTENSVDSKSIKNLEPKIIHGSESMDSGISLDNSYKMDYPEMGLCIIINNKNFHKSTGMTSRSGTDVDAANLRETFRNLKYEVRNKNDLTREEIVELMRDVSKEDHSKRSSFVCVLLSHGEEGIIFGTNGPVDLKKITNFFRGDRCRSLTGKPKLFIIQACRGTELDCGIETDSGVDDDMACHKIPVEADFLYAYSTAPGYYSWRNSKDGSWFIQSLCAMLKQYADKLEFMHILTRVNRKVATEFESFSFDATFHAKKQIPCIVSMLTKELYFYH
Syringe React
Name:
BDBM19728
Synonyms:
(3S)-3-[(1R)-1-[3-(naphthalene-2-amido)-2-oxo-1,2-dihydropyridin-1-yl]-1-phenylacetamido]-4-oxo-5-phenoxypentanoic acid | PEP-1
Type:
Small organic molecule
Emp. Form.:
C35H29N3O7
Mol. Mass.:
603.6207
SMILES:
OC(=O)C[C@H](NC(=O)[C@@H](c1ccccc1)n1cccc(NC(=O)c2ccc3ccccc3c2)c1=O)C(=O)COc1ccccc1 |r|
Structure:
