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Cell Reactant:Caspase-3
Syringe Reactant:BDBM19728
Meas. Tech.:Isothermal Titration Calorimetry
Entry Date:12/19/07
 
ΔG°:-9.67± (kcal/mole)
pH:7.2±n/a
Log10Kb:7.09± 6.40
Temperature:298.15±n/a (K)
ΔH° :n/a
ΔHobs :-2.46±0.0300 (kcal/mole)
Ionic Strength:n/a
Corrected for ΔHioniz:no
Protons Released:n/a
ΔCp :n/a
Stoich. Param.:1.14
ΔS° :0.0200± (kcal/mole-K)
Comments:n/a
 
Citation Aulabaugh, AKapoor, BHuang, XDollings, PHum, WTBanker, AWood, AEllestad, G Biochemical and biophysical characterization of inhibitor binding to caspase-3 reveals induced asymmetry. Biochemistry46:9462-71 (2007) [PubMed]  Article
More Info.:  Get all data from this article ,  ITC RUN data ,  Solution Info ,  Data Fit Method ,  Instrument Info
 
Caspase-3
Source:The DNA sequence encoding the caspase-3 catalytic domain with a C-terminal hexa-His tag was cloned and expressed in Escherichia coli BL21 (DE3) cells.
Purity:>95%
Prep. Method:The cell extracts were further purified by Ni-NTA agarose and gel-filtration columns. The purified caspase-3 showed 2 subunits of 20 and 10 kDa on reduced SDS-PAGE.
Name:Caspase-3
Synonyms:Apopain | CASP-3 | CPP-32 | Caspase 3 | Caspase-3 subunit p12 | Caspase-3 subunit p17 | Cysteine protease CPP32 | SCA-1 | SREBP cleavage activity 1 | Yama protein | caspase-3 preproprotein
Type:Hydrolase; heterotetramer of two heterodimers
Mol. Mass.:31607.55
Organism:Homo sapiens (Human)
Description:n/a
Residue:277
Sequence:
MENTENSVDSKSIKNLEPKIIHGSESMDSGISLDNSYKMDYPEMGLCIIINNKNFHKSTG
MTSRSGTDVDAANLRETFRNLKYEVRNKNDLTREEIVELMRDVSKEDHSKRSSFVCVLLS
HGEEGIIFGTNGPVDLKKITNFFRGDRCRSLTGKPKLFIIQACRGTELDCGIETDSGVDD
DMACHKIPVEADFLYAYSTAPGYYSWRNSKDGSWFIQSLCAMLKQYADKLEFMHILTRVN
RKVATEFESFSFDATFHAKKQIPCIVSMLTKELYFYH
Blast this sequence in BindingDB or PDB
  Blast E-value cutoff:
BDBM19728
Source:n/a
Purity:n/a
Prep. Method:n/a
NameBDBM19728
Synonyms:(3S)-3-[(1R)-1-[3-(naphthalene-2-amido)-2-oxo-1,2-dihydropyridin-1-yl]-1-phenylacetamido]-4-oxo-5-phenoxypentanoic acid | PEP-1
TypeSmall organic molecule
Emp. Form.C35H29N3O7
Mol. Mass.603.6207
SMILESOC(=O)C[C@H](NC(=O)[C@@H](c1ccccc1)n1cccc(NC(=O)c2ccc3ccccc3c2)c1=O)C(=O)COc1ccccc1 |r|
Structure
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Last update November 1, 2007
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