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Cell Reactant:Rho Transcription Termination Factor
Syringe Reactant:BDBM22600
Meas. Tech.:Isothermal Titration Calorimetry
Entry Date:06/14/08
 
ΔG°:-25.1±0.334 (kJ/mole)
pH:7.9±n/a
Log10Kb:4.39± 3.48
Temperature:299.15±n/a (K)
ΔH° :n/a
ΔHobs :-3.30±3.01 (kJ/mole)
Ionic Strength:n/a
Corrected for ΔHioniz:no
Protons Released:n/a
ΔCp :n/a
Stoich. Param.:6
ΔS° : 0.0729±0.0112 (kJ/mole-K)
Comments:The stoichiometry parameter was fixed to six, or for the two site model, two integers with a sum of six.
 
Citation Brogan, APWidger, WRBensadek, DRiba-Garcia, IGaskell, SJKohn, H Development of a technique to determine bicyclomycin-rho binding and stoichiometry by isothermal titration calorimetry and mass spectrometry. J Am Chem Soc127:2741-51 (2005) [PubMed]  Article
More Info.:  Get all data from this article ,  ITC RUN data ,  Solution Info ,  Data Fit Method ,  Instrument Info
 
Rho Transcription Termination Factor
Source:The open reading frame encoding the full-length Escherichia coli Rho protein was PCR amplified and cloned. The protein was overexpressed in E. coli strain BL21 (pLysS).
Purity:99%
Prep. Method:The protein was twice purified over a POROS-HS column (Perseptive Biosystems) then further purified with two successive Sephacryl S-300 (Amersham) sizing columns. The protein at this stage was more than 99% pure as judged by SDS-PAGE.
Name:Rho Transcription Termination Factor
Synonyms:ATP-dependent helicase rho
Type:Enzyme
Mol. Mass.:47006.70
Organism:Escherichia coli (strain K12)
Description:Rho is a hexameric RNA/DNA helicase/translocase.
Residue:419
Sequence:
MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAKSGEDIFGDGVLEILQD
GFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIRPPKEGERYFALLKVNEVN
FDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTARVLDLASPIGRGQRGLIVAPP
KAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTEMQRLVKGEVVASTFDEPASRHV
QVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNTVVPASGKVLTGGVDANALHRPKRF
FGAARNVEEGGSLTIIATALIDTGSKMDEVIYEEFKGTGNMELHLSRKIAEKRVFPAIDY
NRSGTRKEELLTTQEELQKMWILRKIIHPMGEIDAMEFLINKLAMTKTNDDFFEMMKRS
Blast this sequence in BindingDB or PDB
  Blast E-value cutoff:
BDBM22600
Source:n/a
Purity:n/a
Prep. Method:n/a
NameBDBM22600
Synonyms:(1S,6R)-6-hydroxy-5-methylidene-1-[(1S,2S)-1,2,3-trihydroxy-2-methylpropyl]-2-oxa-7,9-diazabicyclo[4.2.2]decane-8,10-dione | Bicyclomycin | CGP 354E | FR 1881 | bicozamycin
TypeAnti-Bacterial Agent
Emp. Form.C12H18N2O7
Mol. Mass.302.2805
SMILESC[C@](O)(CO)[C@H](O)[C@@]12NC(=O)[C@@](O)(NC1=O)C(=C)CCO2 |TLB:10:9:13.14:20.16.19.18,THB:15:14:8.9:20.16.19.18,17:16:13.14:8.9|
Structure
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Last update November 1, 2007
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