BindingDB logo
myBDB logout
Reaction Details
Report a problem with these data
Cell Reactant:ferricytochrome c peroxidase (CcP)
Syringe Reactant:iso-1-cytochrome c (Cc)
Meas. Tech.:ITC
Entry Date:12/15/00
 
ΔG°:-32.8±1.26 (kJ/mole)
pH:6±n/a
Log10Kb:n/a
Temperature:288.15±n/a (K)
ΔH° :-18.1±0.840 (kJ/mole)
ΔHobs :-11.8± (kJ/mole)
Ionic Strength:n/a
Corrected for ΔHioniz:yes
Protons Released:0.6
ΔCp :-0.9072±0.2604 (kJ/mole)
Stoich. Param.:1
ΔS° : 0.0729±0.00700 (kJ/mole-K)
Comments:test the effect of temperature on binding
 
Citation Wang, XPielak, GJ Equilibrium thermodynamics of a physiologically-relevant heme-protein complex. Biochemistry38:16876-81 (1999) [PubMed]  Article
More Info.:  Get all data from this article ,  ITC RUN data ,  Solution Info ,  Instrument Info
 
ferricytochrome c peroxidase (CcP)
Source:recombinant yeast CcP was expressed and purified from E.coli
Purity:n/a
Prep. Method:CcP and Cc were dialyzed (Slide -A_Lyzer membrane) against buffer in the same beaker for > 36 h with three buffer changes
Name:ferricytochrome c peroxidase (CcP)
Synonyms:n/a
Type:Enzyme
Mol. Mass.:40387.06
Organism:Saccharomyces cerevisiae
Description:A0A0D3U836
Residue:362
Sequence:
MTTAVRLLPSLGRTAHKRSLYLFSAAAAAAAAAATFAYSQSHKRSSSSPGGGSNHGWNNW
GKAAALASTTPLVHVASVEKGRSYEDFQKVYNAIALKLREDDEYDNYIGYGPVLVRLAWH
ISGTWDKHDNTGGSYGGTYRFKKEFNDPSNAGLQNGFKFLEPIHKEFPWISSGDLFSLGG
VTAVQEMQGPKIPWRCGRVDTPEDTTPDNGRLPDADKDAGYVRTFFQRLNMNDREVVALM
GAHALGKTHLKNSGYEGPWGAANNVFTNEFYLNLLNEDWKLEKNDANNEQWDSKSGYMML
PTDYSLIQDPKYLSIVKEYANDQDKFFKDFSKAFEKLLENGITFPKDAPSPFIFKTLEEQ
GL
Blast this sequence in BindingDB or PDB
  Blast E-value cutoff:
iso-1-cytochrome c (Cc)
Source:purified from yeast
Purity:n/a
Prep. Method:CcP and Cc were dialyzed (Slide -A_Lyzer membrane) against buffer in the same beaker for > 36 h with three buffer changes
Name:iso-1-cytochrome c (Cc)
Synonyms:n/a
Type:Protein
Mol. Mass.:12191.93
Organism:Saccharomyces cerevisiae
Description:A0A0C6AZG8
Residue:109
Sequence:
MTEFKAGSAKKGATLFKTRCLQCHTVEKGGPHKVGPNLHGIFGRHSGQAEGYSYTDANIK
KNVLWDENNMSEYLTNPKKYIPGTKMAFGGLKKEKDRNDLITYLKKACE
Blast this sequence in BindingDB or PDB
  Blast E-value cutoff:

   
    

Home

|

Search

|

Deposit

|

SiteMap

|

About us

|

Email us

|

Info

 
Last update November 1, 2007
©2000 BindingDB. All rights reserved.