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Cell Reactant:Grb2-SH2
Syringe Reactant:pY317 (phosphotyrosine polypeptide)
Meas. Tech.:ITC
Entry Date:11/29/00
 
ΔG°:-38.6±0.378 (kJ/mole)
pH:7.5±n/a
Log10Kb:6.64± 5.81
Temperature:303.15±n/a (K)
ΔH° :-38.4±0.924 (kJ/mole)
ΔHobs :-38.4± (kJ/mole)
Ionic Strength:n/a
Corrected for ΔHioniz:yes
Protons Released:0
ΔCp :-0.6132±n/a (kJ/mole)
Stoich. Param.:n/a
ΔS° : 0.001±0.00400 (kJ/mole-K)
Comments:n/a
 
Citation McNemar, CSnow, MEWindsor, WTProngay, AMui, PZhang, RDurkin, JLe, HVWeber, PC Thermodynamic and structural analysis of phosphotyrosine polypeptide binding to Grb2-SH2. Biochemistry36:10006-14 (1997) [PubMed]  Article
More Info.:  Get all data from this article ,  ITC RUN data ,  Solution Info ,  Instrument Info
 
Grb2-SH2
Source:DNA construct for the Grb2-SH2 domain was expressed in E.coli
Purity:>98%
Prep. Method:Mono-Q and Mono-S columns
Name:Growth factor receptor-bound protein 2
Synonyms:GRB2 | GRB2 adapter protein | Growth factor receptor-bound protein 2
Type:Protein
Mol. Mass.:25205.04
Organism:Homo sapiens (Human)
Description:P62993
Residue:217
Sequence:
MEAIAKYDFKATADDELSFKRGDILKVLNEECDQNWYKAELNGKDGFIPKNYIEMKPHPW
FFGKIPRAKAEEMLSKQRHDGAFLIRESESAPGDFSLSVKFGNDVQHFKVLRDGAGKYFL
WVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQVPQQPTYVQALFDFDPQEDGELGFRRG
DFIHVMDNSDPNWWKGACHGQTGMFPRNYVTPVNRNV
Blast this sequence in BindingDB or PDB
  Blast E-value cutoff:
pY317 (phosphotyrosine polypeptide)
Source:AnaSpec Inc. (San Jose, CA)
Purity:n/a
Prep. Method:Fmoc solid-phase peptide synthesis
Name:pY317 (phosphotyrosine polypeptide)
Synonyms:n/a
Type:Peptide
Mol. Mass.:708.76
Organism:n/a
Description:phosphotyrosine polypeptide
Residue:6
Sequence:
SYVNVQ
 

   
    

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Last update November 1, 2007
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