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Cell Reactant:Grb2-SH2
Syringe Reactant:pY317 N(+2)A
Meas. Tech.:ITC
Entry Date:11/29/00
 
ΔG°:-19.4±0.336 (kJ/mole)
pH:7.5±n/a
Log10Kb:3.44± n/a
Temperature:293.15±n/a (K)
ΔH° :-8.19±0.0840 (kJ/mole)
ΔHobs :-8.19± (kJ/mole)
Ionic Strength:n/a
Corrected for ΔHioniz:yes
Protons Released:0
ΔCp :n/a
Stoich. Param.:n/a
ΔS° : 0.038±0.00100 (kJ/mole-K)
Comments:n/a
 
Citation McNemar, CSnow, MEWindsor, WTProngay, AMui, PZhang, RDurkin, JLe, HVWeber, PC Thermodynamic and structural analysis of phosphotyrosine polypeptide binding to Grb2-SH2. Biochemistry36:10006-14 (1997) [PubMed]  Article
More Info.:  Get all data from this article ,  ITC RUN data ,  Solution Info ,  Instrument Info
 
Grb2-SH2
Source:DNA construct for the Grb2-SH2 domain was expressed in E.coli
Purity:>98%
Prep. Method:Mono-Q and Mono-S columns
Name:Growth factor receptor-bound protein 2
Synonyms:GRB2 | GRB2 adapter protein | Growth factor receptor-bound protein 2
Type:Protein
Mol. Mass.:25205.04
Organism:Homo sapiens (Human)
Description:P62993
Residue:217
Sequence:
MEAIAKYDFKATADDELSFKRGDILKVLNEECDQNWYKAELNGKDGFIPKNYIEMKPHPW
FFGKIPRAKAEEMLSKQRHDGAFLIRESESAPGDFSLSVKFGNDVQHFKVLRDGAGKYFL
WVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQVPQQPTYVQALFDFDPQEDGELGFRRG
DFIHVMDNSDPNWWKGACHGQTGMFPRNYVTPVNRNV
Blast this sequence in BindingDB or PDB
  Blast E-value cutoff:
pY317 N(+2)A
Source:AnaSpec Inc. (San Jose, CA)
Purity:n/a
Prep. Method:Fmoc solid-phase peptide synthesis
Name:pY317 N(+2)A
Synonyms:n/a
Type:Peptide
Mol. Mass.:665.74
Organism:n/a
Description:alanine substitution of pY317, a phosphotyrosine polypeptide
Residue:6
Sequence:
SYVAVQ
 

   
    

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Last update November 1, 2007
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