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Cell Reactant:HyHEL-10 Mutant (HY58A)
Syringe Reactant:Lysozyme (hen egg)
Meas. Tech.:ITC
Entry Date:12/20/00
 
ΔG°:-43.3± (kJ/mole)
pH:7.2±n/a
Log10Kb:7.34± n/a
Temperature:308±n/a (K)
ΔH° :-79± (kJ/mole)
ΔHobs :-79± (kJ/mole)
Ionic Strength:n/a
Corrected for ΔHioniz:n/a
Protons Released:n/a
ΔCp :-1.09±n/a (kJ/mole)
Stoich. Param.:n/a
ΔS° : -0.116± (kJ/mole-K)
Comments:n/a
 
Citation Nishimiya, YTsumoto, KShiroishi, MYutani, KKumagai, I Thermodynamic consequences of grafting enhanced affinity toward the mutated antigen onto an antibody. The case of anti-lysozyme antibody, HyHEL-10. J Biol Chem275:12813-20 (2000) [PubMed]  Article
More Info.:  Get all data from this article ,  ITC RUN data ,  Solution Info ,  Instrument Info
 
HyHEL-10 Mutant (HY58A)
Source:Described in Rajpal, A., Taylor, M. G., and Kirsch, J. F. (1998) Protein Sci. 7, 1868-1874.
Purity:n/a
Prep. Method:n/a
Name:Lysozyme (hen egg)
Synonyms:D1.3 Fv | D1.3 mAb | hen egg lysozyme (HEL) | hen egg lysozyme(HEL)
Type:Enzyme
Mol. Mass.:16805.63
Organism:Mus musculus (mouse)
Description:P17897
Residue:148
Sequence:
MKALLTLGLLLLSVTAQAKVYNRCELARILKRNGMDGYRGVKLADWVCLAQHESNYNTRA
TNYNRGDRSTDYGIFQINSRYWCNDGKTPRSKNACGINCSALLQDDITAAIQCAKRVVRD
PQGIRAWVAWRTQCQNRDLSQYIRNCGV
Blast this sequence in BindingDB or PDB
  Blast E-value cutoff:
Lysozyme (hen egg)
Source:Seikagaku-kogyo Inc. (Tokyo, Japan)
Purity:n/a
Prep. Method:n/a
Name:Lysozyme (hen egg)
Synonyms:D1.3 Fv | D1.3 mAb | hen egg lysozyme (HEL) | hen egg lysozyme(HEL)
Type:Enzyme
Mol. Mass.:16805.63
Organism:Mus musculus (mouse)
Description:P17897
Residue:148
Sequence:
MKALLTLGLLLLSVTAQAKVYNRCELARILKRNGMDGYRGVKLADWVCLAQHESNYNTRA
TNYNRGDRSTDYGIFQINSRYWCNDGKTPRSKNACGINCSALLQDDITAAIQCAKRVVRD
PQGIRAWVAWRTQCQNRDLSQYIRNCGV
Blast this sequence in BindingDB or PDB
  Blast E-value cutoff:

   
    

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Last update November 1, 2007
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