BindingDB logo
myBDB logout
Reaction Details
Report a problem with these data
Cell Reactant:DNA
Syringe Reactant:vnd/NK-2 HD
Meas. Tech.:Isothermal Titration Calorimetry
Entry Date:08/31/01
 
ΔG°:-46.0±0.418 (kJ/mole)
pH:7.4±0.01
Log10Kb:8.08± 7
Temperature:298.15±n/a (K)
ΔH° :n/a
ΔHobs :-27.6±2.09 (kJ/mole)
Ionic Strength:50±n/a
Corrected for ΔHioniz:yes
Protons Released:n/a
ΔCp :1.045±0.1672 (kJ/mole)
Stoich. Param.:1
ΔS° :n/a
Comments:n/a
 
Citation Gonzalez, MWeiler, SFerretti, JAGinsburg, A The vnd/NK-2 homeodomain: thermodynamics of reversible unfolding and DNA binding for wild-type and with residue replacements H52R and H52R/T56W in helix III. Biochemistry40:4923-31 (2001) [PubMed]  Article
More Info.:  Get all data from this article ,  ITC RUN data ,  Solution Info ,  Data Fit Method ,  Instrument Info
 
DNA
Source:purchased
Purity:n/a
Prep. Method:n/a
Name:DNA
Synonyms:n/a
Type:Duplex DNA
Mol. Mass.:6891.48
Organism:Synthetic
Description:n/a
Residue:72
Sequence:
SINGLESTRANDASEDNATGTGTCAAGTGGCTGTAGANDITSCMPLEMENTARYCTACAG
CCACTTGACACA
Blast this sequence in BindingDB or PDB
  Blast E-value cutoff:
vnd/NK-2 HD
Source:own production
Purity:100
Prep. Method:overproduction in E. coli, His-tag purified, dialyzed, liophylized, resuspended and dialyzed in working buffer
Name:vnd/NK-2 HD
Synonyms:HD(wt)
Type:homeodomain protein
Mol. Mass.:358.43
Organism:n/a
Description:n/a
Residue:3
Sequence:
n/a
 

   
    

Home

|

Search

|

Deposit

|

SiteMap

|

About us

|

Email us

|

Info

 
Last update November 1, 2007
©2000 BindingDB. All rights reserved.