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TargetUbiquitin C-terminal Hydrolase L3 (UCH-L3)
LigandBDBM65524
Substrate/Competitorn/a
Meas. Tech.Fluorometric Ub-AMC Hydrolysis Assay
pH7.5±n/a
Temperature298.15±n/a K
IC50>114000±n/a nM
Commentsextracted
Citation Ott, CABaljinnyam, BZakharov, AVJadhav, ASimeonov, AZhuang, Z Cell Lysate-Based AlphaLISA Deubiquitinase Assay Platform for Identification of Small Molecule Inhibitors. ACS Chem Biol12:2399-2407 (2017) [PubMed]  Article
More Info.:Get all data from this article,  Assay Method
 
Ubiquitin C-terminal Hydrolase L3 (UCH-L3)
Name:Ubiquitin C-terminal Hydrolase L3 (UCH-L3)
Synonyms:Ubiquitin thioesterase L3
Type:Hydrolase; protease
Mol. Mass.:26168.69
Organism:Homo sapiens (Human)
Description:Human recombinant UCH-L3 was purchased from Boston Biochem, Inc.(Cambridge, MA).
Residue:230
Sequence:
MEGQRWLPLEANPEVTNQFLKQLGLHPNWQFVDVYGMDPELLSMVPRPVCAVLLLFPITE
KYEVFRTEEEEKIKSQGQDVTSSVYFMKQTISNACGTIGLIHAIANNKDKMHFESGSTLK
KFLEESVSMSPEERARYLENYDAIRVTHETSAHEGQTEAPSIDEKVDLHFIALVHVDGHL
YELDGRKPFPINHGETSDETLLEDAIEVCKKFMERDPDELRFNAIALSAA
Blast this sequence in BindingDB or PDB
  Blast E-value cutoff:
BDBM65524
NameBDBM65524
Synonyms:(-)-Aricine | Heterophylline (VAN) | Quinovatine
TypeSmall organic molecule
Emp. Form.C22H26N2O4
Mol. Mass.382.4528
SMILESCOC(=O)C1=CO[C@@H](C)[C@@H]2CN3CCc4c([nH]c5cc(OC)ccc45)[C@@H]3C[C@H]12 |t:4|
Structure
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