Reaction Details Report a problem with these data
Target
Macrophage colony-stimulating factor 1 receptor [538-678,753-922]
Ligand
BDBM7393
Substrate
cFMS -Tie-2 Chimera
Meas. Tech.
cFMs Autophosphorylation Fluorescence Polarization End Point Assay.
pH
7.5±n/a
Temperature
295.15±n/a K
IC50
4500±500 nM
Citation
 Schalk-Hihi, C; Ma, HC; Struble, GT; Bayoumy, S; Williams, R; Devine, E; Petrounia, IP; Mezzasalma, T; Zeng, L; Schubert, C; Grasberger, B; Springer, BA; Deckman, IC Protein engineering of the colony-stimulating factor-1 receptor kinase domain for structural studies. J Biol Chem 282:4085-93 (2007) [PubMed]  Article 
Target
Name:
Macrophage colony-stimulating factor 1 receptor [538-678,753-922]
Synonyms:
6His.FMS.538-922(TIE2) | CSF1R | CSF1R_HUMAN | FMS | Tyrosine Kinase CSF1-R Mutant (Tie2 KID Chimera) | cFMS -Tie-2 Chimera
Type:
Other Protein Type
Mol. Mass.:
37574.75
Organism:
Homo sapiens (Human)
Description:
Recombinant protein contains FMS cytoplasmic domain (FMS538-922.6His), in which FMS KID was replaced with Tie-2 KID.
Residue:
333
Sequence:
GVDYKYKQKPKYQVRWKIIESYEGNSYTFIDPTQLPYNEKWEFPRNNLQFGKTLGAGAFGKVVEATAFGLGKEDAVLKVAVKMLKSTAHADEKEALMSELKIMSHLGQHENIVNLLGACTHGGPVLVITEYCCYGDLLNFLRRKSRVLETDPAFAIANSTASTRDLLHFSSQVAQGMAFLASKNCIHRDVAARNVLLTNGHVAKIGDFGLARDIMNDSNYIVKGNARLPVKWMAPESIFDCVYTVQSDVWSYGILLWEIFSLGLNPYPGILVNSKFYKLVKDGYQMAQPAFAPKNIYSIMQACWALEPTHRPTFQQICSFLQEQAQEDRRERD
  
Inhibitor
Name:
BDBM7393
Synonyms:
3-[(2Z,3E)-3-(hydroxyimino)-2,3-dihydro-1H-indol-2-ylidene]-2,3-dihydro-1H-indol-2-one | CHEMBL397695 | Indirubin-3 -monoxime | Indirubin-3-monoxime | Indirubin-3monoxime | indirubin deriv. 7h
Type:
n/a
Emp. Form.:
C16H11N3O2
Mol. Mass.:
277.2774
SMILES:
O=Nc1c([nH]c2ccccc12)C1C(=O)Nc2ccccc12
Structure:
Search PDB for entries with ligand similarity:
Substrate
Name:
Macrophage colony-stimulating factor 1 receptor [538-678,753-922]
Synonyms:
6His.FMS.538-922(TIE2) | CSF1R | CSF1R_HUMAN | FMS | Tyrosine Kinase CSF1-R Mutant (Tie2 KID Chimera) | cFMS -Tie-2 Chimera
Type:
Other Protein Type
Mol. Mass.:
37574.75
Organism:
Homo sapiens (Human)
Description:
Recombinant protein contains FMS cytoplasmic domain (FMS538-922.6His), in which FMS KID was replaced with Tie-2 KID.
Residue:
333
Sequence:
GVDYKYKQKPKYQVRWKIIESYEGNSYTFIDPTQLPYNEKWEFPRNNLQFGKTLGAGAFGKVVEATAFGLGKEDAVLKVAVKMLKSTAHADEKEALMSELKIMSHLGQHENIVNLLGACTHGGPVLVITEYCCYGDLLNFLRRKSRVLETDPAFAIANSTASTRDLLHFSSQVAQGMAFLASKNCIHRDVAARNVLLTNGHVAKIGDFGLARDIMNDSNYIVKGNARLPVKWMAPESIFDCVYTVQSDVWSYGILLWEIFSLGLNPYPGILVNSKFYKLVKDGYQMAQPAFAPKNIYSIMQACWALEPTHRPTFQQICSFLQEQAQEDRRERD