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Target
Diacylglycerol O-acyltransferase 1
Ligand
BDBM20722
Substrate
BDBM20717
Meas. Tech.
In Vitro FlashPlate Assay
pH
7.5±n/a
Temperature
295.15±n/a K
IC50
21±n/a nM
Citation
 Zhao, G; Souers, AJ; Voorbach, M; Falls, HD; Droz, B; Brodjian, S; Lau, YY; Iyengar, RR; Gao, J; Judd, AS; Wagaw, SH; Ravn, MM; Engstrom, KM; Lynch, JK; Mulhern, MM; Freeman, J; Dayton, BD; Wang, X; Grihalde, N; Fry, D; Beno, DW; Marsh, KC; Su, Z; Diaz, GJ; Collins, CA; Sham, H; Reilly, RM; Brune, ME; Kym, PR Validation of diacyl glycerolacyltransferase I as a novel target for the treatment of obesity and dyslipidemia using a potent and selective small molecule inhibitor. J Med Chem 51:380-3 (2008) [PubMed]  Article 
Target
Name:
Diacylglycerol O-acyltransferase 1
Synonyms:
DGAT1_MOUSE | Dgat | Dgat1 | Diacyl Glycerolacyltransferase 1 (DGAT-1) | Diacylglycerol O-acyltransferase 1 (DGAT1) | Diglyceride acyltransferase
Type:
Enzyme
Mol. Mass.:
56810.61
Organism:
Mus musculus (mouse)
Description:
In this assay, recombinant mouse DGAT-1 containing an N-terminal His6-epitope tag was produced in the baculovirus expression system.
Residue:
498
Sequence:
MGDRGGAGSSRRRRTGSRVSVQGGSGPKVEEDEVRDAAVSPDLGAGGDAPAPAPAPAHTRDKDGRTSVGDGYWDLRCHRLQDSLFSSDSGFSNYRGILNWCVVMLILSNARLFLENLIKYGILVDPIQVVSLFLKDPYSWPAPCVIIASNIFVVAAFQIEKRLAVGALTEQMGLLLHVVNLATIICFPAAVALLVESITPVGSVFALASYSIMFLKLYSYRDVNLWCRQRRVKAKAVSTGKKVSGAAAQQAVSYPDNLTYRDLYYFIFAPTLCYELNFPRSPRIRKRFLLRRVLEMLFFTQLQVGLIQQWMVPTIQNSMKPFKDMDYSRIIERLLKLAVPNHLIWLIFFYWFFHSCLNAVAELLQFGDREFYRDWWNAESVTYFWQNWNIPVHKWCIRHFYKPMLRHGSSKWVARTGVFLTSAFFHEYLVSVPLRMFRLWAFTAMMAQVPLAWIVGRFFQGNYGNAAVWVTLIIGQPVAVLMYVHDYYVLNYDAPVGV
  
Inhibitor
Name:
BDBM20722
Synonyms:
(1R,2R)-2-({4-[4-({[2-fluoro-5-(trifluoromethyl)phenyl]carbamoyl}amino)phenyl]phenyl}carbonyl)cyclopentane-1-carboxylic acid | Urea-based Analogue, 4f
Type:
Small organic molecule
Emp. Form.:
C27H22F4N2O4
Mol. Mass.:
514.4682
SMILES:
OC(=O)[C@@H]1CCC[C@H]1C(=O)c1ccc(cc1)-c1ccc(NC(=O)Nc2cc(ccc2F)C(F)(F)F)cc1 |r|
Structure:
Search PDB for entries with ligand similarity:
Substrate
Name:
BDBM20717
Synonyms:
(1-decanoyloxy-3-hydroxypropan-2-yl) decanoate | 1,2-didecanoyl-sn-glycerol | 1-(decanoyloxy)-3-hydroxypropan-2-yl decanoate | didecanoyl glycerol
Type:
n/a
Emp. Form.:
C23H44O5
Mol. Mass.:
400.5925
SMILES:
CCCCCCCCCC(=O)OCC(CO)OC(=O)CCCCCCCCC
Structure:
Search PDB for entries with ligand similarity: