Reaction Details Report a problem with these data
Target
Neprilysin
Ligand
BDBM21639
Substrate
BDBM21631
Meas. Tech.
In Vitro Inhibition of NEP
pH
7.4±n/a
Temperature
310.15±n/a K
Ki
1.8±0.2 nM
Km
37000±n/a nM
Citation
 Inguimbert, N; Coric, P; Poras, H; Meudal, H; Teffot, F; Fournié-Zaluski, MC; Roques, BP Toward an optimal joint recognition of the S1' subsites of endothelin converting enzyme-1 (ECE-1), angiotensin converting enzyme (ACE), and neutral endopeptidase (NEP). J Med Chem 45:1477-86 (2002) [PubMed]  Article 
Target
Name:
Neprilysin
Synonyms:
Atriopeptidase | CD10 antigen | CD_antigen=CD10 | Enkephalinase | MME | NEP | NEP_RABIT | Neutral Endopeptidase | Neutral endopeptidase 24.11
Type:
Enzyme
Mol. Mass.:
85570.75
Organism:
Oryctolagus cuniculus (rabbit)
Description:
NEP was purified to homogeneity from rabbit kidney.
Residue:
750
Sequence:
MGRSESQMDITDINTPKPKKKQRWTPLEISLSVLVLLLTVIAVTMIALYATYDDGICKSSDCIKSAARLIQNMDATAEPCTDFFKYACGGWLKRNVIPETSSRYSNFDILRDELEVILKDVLQEPKTEDIVAVQKAKTLYRSCVNETAIDSRGGQPLLKLLPDVYGWPVATQNWEQTYGTSWSAEKSIAQLNSNYGKKVLINFFVGTDDKNSMNHIIHIDQPRLGLPSRDYYECTGIYKEACTAYVDFMIAVAKLIRQEEGLPIDENQISVEMNKVMELEKEIANATTKSEDRNDPMLLYNKMTLAQIQNNFSLEINGKPFSWSNFTNEIMSTVNINIPNEEDVVVYAPEYLIKLKPILTKYFPRDFQNLFSWRFIMDLVSSLSRTYKDSRNAFRKALYGTTSESATWRRCANYVNGNMENAVGRLYVEAAFAGESKHVVEDLIAQIREVFIQTLDDLTWMDAETKKKAEEKALAIKERIGYPDDIVSNDNKLNNEYLELNYKEDEYFENIIQNLKFSQSKQLKKLREKVDKDEWITGAAIVNAFYSSGRNQIVFPAGILQPPFFSAQQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDGDLVDWWTQQSANNFKEQSQCMVYQYGNFSWDLAGGQHLNGINTLGENIADNGGIGQAYRAYQNYVKKNGEEKLLPGIDLNHKQLFFLNFAQVWCGTYRPEYAVNSIKTDVHSPGNFRIIGSLQNSVEFSEAFQCPKNSYMNPEKKCRVW
  
Inhibitor
Name:
BDBM21639
Synonyms:
(2S)-2-[2-(2,3-dihydro-1H-inden-1-yl)-3-sulfanylpropanamido]-3-(1H-indol-3-yl)propanoic acid | Mercaptoacyl amino acid compound, (rac)-13 | racemic mixture
Type:
Small organic molecule
Emp. Form.:
C23H24N2O3S
Mol. Mass.:
408.513
SMILES:
OC(=O)[C@H](Cc1c[nH]c2ccccc12)NC(=O)C(CS)C1CCc2ccccc12 |r|
Structure:
Search PDB for entries with ligand similarity:
Substrate
Name:
BDBM21631
Synonyms:
DGPA | Dansyl-Gly-(p-NO2)Phe-beta-Ala
Type:
fluorogenic substrate
Emp. Form.:
n/a
Mol. Mass.:
n/a
SMILES:
n/a
Structure: