Reaction Details Report a problem with these data
Target
Homoisocitrate dehydrogenase, mitochondrial
Ligand
BDBM23216
Substrate
BDBM23214
Meas. Tech.
Enzyme Inhibition Assay
pH
7.8±n/a
Temperature
309.15±n/a K
Ki
12000±n/a nM
Km
18000±n/a nM
kcat
17±n/a 1/sec
Comments
Uncompetitive inhibitor.
Citation
 Yamamoto, T; Eguchi, T Thiahomoisocitrate: a highly potent inhibitor of homoisocitrate dehydrogenase involved in the alpha-aminoadipate pathway. Bioorg Med Chem 16:3372-6 (2008) [PubMed]  Article 
Target
Name:
Homoisocitrate dehydrogenase, mitochondrial
Synonyms:
Homoisocitrate Dehydrogenase (HICDH) | Homoisocitrate dehydrogenase, mitochondrial | LYS10 | LYS11 | LYS12 | LYS12_YEAST | ScHICDH
Type:
Enzyme
Mol. Mass.:
40072.49
Organism:
Saccharomyces cerevisiae
Description:
n/a
Residue:
371
Sequence:
MFRSVATRLSACRGLASNAARKSLTIGLIPGDGIGKEVIPAGKQVLENLNSKHGLSFNFIDLYAGFQTFQETGKALPDETVKVLKEQCQGALFGAVQSPTTKVEGYSSPIVALRREMGLFANVRPVKSVEGEKGKPIDMVIVRENTEDLYIKIEKTYIDKATGTRVADATKRISEIATRRIATIALDIALKRLQTRGQATLTVTHKSNVLSQSDGLFREICKEVYESNKDKYGQIKYNEQIVDSMVYRLFREPQCFDVIVAPNLYGDILSDGAAALVGSLGVVPSANVGPEIVIGEPCHGSAPDIAGKGIANPIATIRSTALMLEFLGHNEAAQDIYKAVDANLREGSIKTPDLGGKASTQQVVDDVLSRL
  
Inhibitor
Name:
BDBM23216
Synonyms:
3-[(carboxymethyl)sulfanyl]-2-oxopropanoic acid | Homoisocitrate thia analogue, 4
Type:
Small organic molecule
Emp. Form.:
C5H6O5S
Mol. Mass.:
178.163
SMILES:
OC(=O)CSCC(=O)C(O)=O
Structure:
Search PDB for entries with ligand similarity:
Substrate
Name:
BDBM23214
Synonyms:
(-)-homoisocitric acid | (1R,2S)-1-hydroxybutane-1,2,4-tricarboxylic acid | (2R,3S)-Homoisocitrate | Homoisocitrate
Type:
Small organic molecule
Emp. Form.:
C7H10O7
Mol. Mass.:
206.1501
SMILES:
[H][C@](O)(C(O)=O)[C@]([H])(CCC(O)=O)C(O)=O |r|
Structure:
Search PDB for entries with ligand similarity: