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Target
Peptidyl-prolyl cis-trans isomerase FKBP1A
Ligand
BDBM23320
Substrate
BDBM23318
Meas. Tech.
Peptidyl Prolyl Isomerase (PPIase) Inibition Assay
pH
8±n/a
Temperature
273.15±n/a K
Ki
310±n/a nM
Km
520000±80000 nM
kcat
344±26 1/sec
Citation
 Wu, YQ; Wilkinson, DE; Limburg, D; Li, JH; Sauer, H; Ross, D; Liang, S; Spicer, D; Valentine, H; Fuller, M; Guo, H; Howorth, P; Soni, R; Chen, Y; Steiner, JP; Hamilton, GS Synthesis of ketone analogues of prolyl and pipecolyl ester FKBP12 ligands. J Med Chem 45:3558-68 (2002) [PubMed]  Article 
Target
Name:
Peptidyl-prolyl cis-trans isomerase FKBP1A
Synonyms:
12 kDa FKBP | FK506-binding protein 1A | FKB1A_BOVIN | FKBP-12 | FKBP1 | FKBP1A | Immunophilin FKBP12 | PPIase | Rotamase
Type:
Isomerase
Mol. Mass.:
11911.51
Organism:
Bos taurus (bovine)
Description:
The enzyme was purified from calf thymus. The collected protein was further applied to Sephacryl column, and the fraction contained high PPIase activity was pooled.
Residue:
108
Sequence:
MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKPFKFVLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPNATLIFDVELLKLE
  
Inhibitor
Name:
BDBM23320
Synonyms:
1-cyclohexyl-2-[(2S)-2-(5-phenylpentanoyl)pyrrolidin-1-yl]ethane-1,2-dione | N-Glyoxylprolyl ketone, 5c
Type:
Small organic molecule
Emp. Form.:
C23H31NO3
Mol. Mass.:
369.4971
SMILES:
O=C(CCCCc1ccccc1)[C@@H]1CCCN1C(=O)C(=O)C1CCCCC1 |r|
Structure:
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Substrate
Name:
BDBM23318
Synonyms:
3-{[(1S)-1-{[(2S)-4-methyl-1-[(2S)-2-{[(1S)-1-[(4-nitrophenyl)carbamoyl]-2-phenylethyl]carbamoyl}pyrrolidin-1-yl]-1-oxopentan-2-yl]carbamoyl}ethyl]carbamoyl}propanoic acid | N-Succinyl-L-alanyl-L-Leucyl-L-prolyl-L-phenylalanine 4-nitroanilide | N-succinyl Ala-Leu-Pro-Phe p-nitroanilide | Suc-ALPF-pNA
Type:
Colorimetric substrate
Emp. Form.:
C33H42N6O9
Mol. Mass.:
666.7214
SMILES:
CC(C)C[C@H](NC(=O)[C@H](C)NC(=O)CCC(O)=O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](Cc1ccccc1)C(=O)Nc1ccc(cc1)[N+]([O-])=O |r|
Structure:
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