Reaction Details Report a problem with these data
Target
Bcl-2-like protein 1
Ligand
BDBM25542
Substrate
Bak-BH3 peptide
Meas. Tech.
Fluorescence Polarization Assay
pH
8±n/a
Temperature
295.15±n/a K
IC50
22000±n/a nM
Citation
 Bernardo, PH; Wan, KF; Sivaraman, T; Xu, J; Moore, FK; Hung, AW; Mok, HY; Yu, VC; Chai, CL Structure-activity relationship studies of phenanthridine-based Bcl-XL inhibitors. J Med Chem 51:6699-710 (2008) [PubMed]  Article 
Target
Name:
Bcl-2-like protein 1
Synonyms:
Anti-apoptotic Bcl-2 protein | Apoptosis Regulator Bcl-xL | Apoptosis regulator Bcl-X | B2CL1_HUMAN | BCL2-like 1 isoform 1 | BCL2L | BCL2L1 | BCLX | Bcl-2-like protein 1 (Bcl-XL) | Bcl-X | Bcl-xL/Bcl-2-binding component 3 | Bcl2-L-1 | Bcl2-antagonist of cell death (BAD)
Type:
Mitochondrion membrane; Single-pass membrane protein
Mol. Mass.:
26039.60
Organism:
Homo sapiens (Human)
Description:
n/a
Residue:
233
Sequence:
MSQSNRELVVDFLSYKLSQKGYSWSQFSDVEENRTEAPEGTESEMETPSAINGNPSWHLADSPAVNGATGHSSSLDAREVIPMAAVKQALREAGDEFELRYRRAFSDLTSQLHITPGTAYQSFEQVVNELFRDGVNWGRIVAFFSFGGALCVESVDKEMQVLVSRIAAWMATYLNDHLEPWIQENGGWDTFVELYGNNAAAESRKGQERFNRWFLTGMTVAGVVLLGSLFSRK
  
Inhibitor
Name:
BDBM25542
Synonyms:
7,8-dihydroxy-2-(2-hydroxyethyl)-5-methylphenanthridin-5-ium chloride | chelerythrine analogue, 44
Type:
Small organic molecule
Emp. Form.:
C16H16NO3
Mol. Mass.:
270.3026
SMILES:
Cn1cc2c(ccc(O)c2=[OH+])c2cc(CCO)ccc12
Structure:
Search PDB for entries with ligand similarity:
Substrate
Name:
Bak-BH3 peptide
Synonyms:
F-BakBH3
Type:
Fluorescein-labeled peptide
Mol. Mass.:
1724.92
Organism:
n/a
Description:
Derived from human apoptosis regulator BAK (residues 72-87).
Residue:
16
Sequence:
GQVGRQLAIIGDDINR