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TargetB-RAF V600E
LigandBDBM25617
Substrate/Competitorbiotin-MEK
Meas. Tech.In Vitro BRAF Kinase Assay
pH7±n/a
Temperature295.15±n/a K
IC50 13±n/a nM
Citation Tsai, JLee, JTWang, WZhang, JCho, HMamo, SBremer, RGillette, SKong, JHaass, NKSproesser, KLi, LSmalley, KSFong, DZhu, YLMarimuthu, ANguyen, HLam, BLiu, JCheung, IRice, JSuzuki, YLuu, CSettachatgul, CShellooe, RCantwell, JKim, SHSchlessinger, JZhang, KYWest, BLPowell, BHabets, GZhang, CIbrahim, PNHirth, PArtis, DRHerlyn, MBollag, G Discovery of a selective inhibitor of oncogenic B-Raf kinase with potent antimelanoma activity. Proc Natl Acad Sci U S A105:3041-6 (2008) [PubMed]  Article
More Info.:Get all data from this article,   Solution Info,  Assay Method
 
B-RAF V600E
Name:B-RAF V600E
Synonyms:B-Raf (V600E) | B-Raf Protein Kinase Mutant (V600E) | B-Raf proto-oncogene serine/threonine-protein kinase | BRAF (V600E) | BRAF mutant V600E | P15056 | Protein mono-ADP-ribosyltransferase (PARP3) | RAF serine/threonine protein kinase (V600E) | Serine/threonine-protein kinase B-raf (V600E) | Serine/threonine-protein kinase B-raf (V600E) | V-RAF murine sarcoma viral oncogene homologue B1 mutant (BRAF V600E)
Type:n/a
Mol. Mass.:84474.98
Organism:Homo sapiens (Human)
Description:P15056 V600E
Residue:766
Sequence:
MAALSGGGGGGAEPGQALFNGDMEPEAGAGAGAAASSAADPAIPEEVWNIKQMIKLTQEH
IEALLDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLESLGNGTDFSVSSSASMDTV
TSSSSSSLSVLPSSLSVFQNPTDVARSNPKSPQKPIVRVFLPNKQRTVVPARCGVTVRDS
LKKALMMRGLIPECCAVYRIQDGEKKPIGWDTDISWLTGEELHVEVLENVPLTTHNFVRK
TFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDQLDLLFVSKFFEHHPI
PQEEASLAETALTSGSSPSAPASDSIGPQILTSPSPSKSIPIPQPFRPADEDHRNQFGQR
DRSSSAPNVHINTIEPVNIDDLIRDQGFRGDGGSTTGLSATPPASLPGSLTNVKALQKSP
GPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDV
AVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHH
LHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATE
KSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNIN
NRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARS
LPKIHRSASEPSLNRAGFQTEDFSLYACASPKTPIQAGGYGAFPVH
Blast this sequence in BindingDB or PDB
  Blast E-value cutoff:
BDBM25617
NameBDBM25617
Synonyms:N-[3-({5-chloro-1H-pyrrolo[2,3-b]pyridin-3-yl}carbonyl)-2,4-difluorophenyl]propane-1-sulfonamide | PLX4720
TypeSmall organic molecule
Emp. Form.C17H14ClF2N3O3S
Mol. Mass.413.826
SMILESCCCS(=O)(=O)Nc1ccc(F)c(C(=O)c2c[nH]c3ncc(Cl)cc23)c1F
Structure
Search PDB for entries with ligand similarity:Similarity to this molecule at least:
biotin-MEK
Name:biotin-MEK
Synonyms:n/a
Type:biotinylated substrate
Mol. Mass.:358.43
Organism:n/a
Description:Perkin-Elmer s AlphaScreen assay kit.
Residue:3
Sequence:
n/a