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Target
Gamma-aminobutyric acid receptor subunit alpha-1 [28-456]/beta-3 [26-473]/gamma-2 [40-467]
Ligand
BDBM25883
Substrate
BDBM25878
Meas. Tech.
Radioligand Binding Assay
pH
7.4±n/a
Temperature
277.15±n/a K
Ki
287±n/a nM
Citation
 Han, D; Holger Försterling, F; Li, X; Deschamps, JR; Parrish, D; Cao, H; Rallapalli, S; Clayton, T; Teng, Y; Majumder, S; Sankar, S; Roth, BL; Sieghart, W; Furtmuller, R; Rowlett, JK; Weed, MR; Cook, JM A study of the structure-activity relationship of GABA(A)-benzodiazepine receptor bivalent ligands by conformational analysis with low temperature NMR and X-ray analysis. Bioorg Med Chem 16:8853-62 (2008) [PubMed]  Article 
Target
Name:
Gamma-aminobutyric acid receptor subunit alpha-1 [28-456]/beta-3 [26-473]/gamma-2 [40-467]
Synonyms:
GABAA Receptor, alpha-1, beta-3, gamma-2
Type:
Protein Complex
Mol. Mass.:
n/a
Description:
Cell membranes from HEK cells transfected with GABAA beta-3, gamma-2, and alpha-1 were used in binding assays.
Components:
This complex has 3 components.
Component 1
Name:
Gamma-aminobutyric acid receptor subunit gamma-2 [40-467]
Synonyms:
GABA(A) receptor subunit gamma-2 | GABAA Receptor Subunit gamma-2 | GABRG2 | GBRG2_HUMAN | Gamma-aminobutyric acid receptor subunit gamma-2
Type:
Ionic channel
Mol. Mass.:
49862.76
Organism:
Homo sapiens (Human)
Description:
P18507[40-467]
Residue:
428
Sequence:
QKSDDDYEDYASNKTWVLTPKVPEGDVTVILNNLLEGYDNKLRPDIGVKPTLIHTDMYVNSIGPVNAINMEYTIDIFFAQTWYDRRLKFNSTIKVLRLNSNMVGKIWIPDTFFRNSKKADAHWITTPNRMLRIWNDGRVLYTLRLTIDAECQLQLHNFPMDEHSCPLEFSSYGYPREEIVYQWKRSSVEVGDTRSWRLYQFSFVGLRNTTEVVKTTSGDYVVMSVYFDLSRRMGYFTIQTYIPCTLIVVLSWVSFWINKDAVPARTSLGITTVLTMTTLSTIARKSLPKVSYVTAMDLFVSVCFIFVFSALVEYGTLHYFVSNRKPSKDKDKKKKNPAPTIDIRPRSATIQMNNATHLQERDEEYGYECLDGKDCASFFCCFEDCRTGAWRHGRIHIRIAKMDSYARIFFPTAFCLFNLVYWVSYLYL
  
Component 2
Name:
Gamma-aminobutyric acid receptor subunit beta-3 [26-473]
Synonyms:
GABA(A) receptor subunit beta-3 | GABAA Receptor Subunit beta-3 | GABRB3 | GBRB3_HUMAN | Gamma-aminobutyric acid receptor subunit beta-3
Type:
Ionic channel
Mol. Mass.:
51609.41
Organism:
Homo sapiens (Human)
Description:
P28472[26-473]
Residue:
448
Sequence:
QSVNDPGNMSFVKETVDKLLKGYDIRLRPDFGGPPVCVGMNIDIASIDMVSEVNMDYTLTMYFQQYWRDKRLAYSGIPLNLTLDNRVADQLWVPDTYFLNDKKSFVHGVTVKNRMIRLHPDGTVLYGLRITTTAACMMDLRRYPLDEQNCTLEIESYGYTTDDIEFYWRGGDKAVTGVERIELPQFSIVEHRLVSRNVVFATGAYPRLSLSFRLKRNIGYFILQTYMPSILITILSWVSFWINYDASAARVALGITTVLTMTTINTHLRETLPKIPYVKAIDMYLMGCFVFVFLALLEYAFVNYIFFGRGPQRQKKLAEKTAKAKNDRSKSESNRVDAHGNILLTSLEVHNEMNEVSGGIGDTRNSAISFDNSGIQYRKQSMPREGHGRFLGDRSLPHKKTHLRRRSSQLKIKIPDLTDVNAIDRWSRIVFPFTFSLFNLVYWLYYVN
  
Component 3
Name:
Gamma-aminobutyric acid receptor subunit alpha-1 [28-456]
Synonyms:
GABA(A) receptor subunit alpha-1 | GABAA Receptor Subunit alpha-1 | GABRA1 | GBRA1_HUMAN | Gamma-aminobutyric acid receptor subunit alpha-1
Type:
Ionic channel
Mol. Mass.:
48807.81
Organism:
Homo sapiens (Human)
Description:
P14867[28-456]
Residue:
429
Sequence:
QPSLQDELKDNTTVFTRILDRLLDGYDNRLRPGLGERVTEVKTDIFVTSFGPVSDHDMEYTIDVFFRQSWKDERLKFKGPMTVLRLNNLMASKIWTPDTFFHNGKKSVAHNMTMPNKLLRITEDGTLLYTMRLTVRAECPMHLEDFPMDAHACPLKFGSYAYTRAEVVYEWTREPARSVVVAEDGSRLNQYDLLGQTVDSGIVQSSTGEYVVMTTHFHLKRKIGYFVIQTYLPCIMTVILSQVSFWLNRESVPARTVFGVTTVLTMTTLSISARNSLPKVAYATAMDWFIAVCYAFVFSALIEFATVNYFTKRGYAWDGKSVVPEKPKKVKDPLIKKNNTYAPTATSYTPNLARGDPGLATIAKSATIEPKEVKPETKPPEPKKTFNSVSKIDRLSRIAFPLLFGIFNLVYWATYLNREPQLKAPTPHQ
  
Inhibitor
Name:
BDBM25883
Synonyms:
ethyl 12-ethynyl-9-phenyl-2,4,8-triazatricyclo[8.4.0.0^{2,6}]tetradeca-1(10),3,5,8,11,13-hexaene-5-carboxylate | imidazobenzodiazepine, 6
Type:
Small organic molecule
Emp. Form.:
C22H17N3O2
Mol. Mass.:
355.3893
SMILES:
CCOC(=O)c1ncn-2c1CN=C(c1ccccc1)c1cc(ccc-21)C#C |t:12|
Structure:
Search PDB for entries with ligand similarity:
Substrate
Name:
BDBM25878
Synonyms:
5-(2-fluorophenyl)-1-methyl-7-nitro-2,3-dihydro-1H-1,4-benzodiazepin-2-one | CHEMBL13280 | Flunitrazepam | [3H]Rohypnol | [3H]flunitrazepam
Type:
radiolabeled ligand
Emp. Form.:
C16H12FN3O3
Mol. Mass.:
313.2832
SMILES:
CN1c2ccc(cc2C(=NCC1=O)c1ccccc1F)[N+]([O-])=O |c:9|
Structure:
Search PDB for entries with ligand similarity: