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Target
Cell division cycle 7-related protein kinase [58-574]/Protein DBF4 homolog A
Ligand
BDBM27433
Substrate
His6-tagged MCM2
Meas. Tech.
Radioactive Kinase Assay
pH
7.5±n/a
Temperature
310.15±n/a K
Ki
5±n/a nM
IC50
50±n/a nM
Citation
 Zhao, C; Tovar, C; Yin, X; Xu, Q; Todorov, IT; Vassilev, LT; Chen, L Synthesis and evaluation of pyrido-thieno-pyrimidines as potent and selective Cdc7 kinase inhibitors. Bioorg Med Chem Lett 19:319-23 (2009) [PubMed]  Article 
Target
Name:
Cell division cycle 7-related protein kinase [58-574]/Protein DBF4 homolog A
Synonyms:
Cdc7 Kinase | Cdc7/DBF4
Type:
Protein Complex
Mol. Mass.:
n/a
Description:
n/a
Components:
This complex has 2 components.
Component 1
Name:
Cell division cycle 7-related protein kinase [58-574]
Synonyms:
CDC7 | CDC7L1 | CDC7_HUMAN | Cdc7 Kinase | Cell division cycle 7-related protein kinase
Type:
Serine/threonine-protein kinase
Mol. Mass.:
57470.88
Organism:
Homo sapiens (Human)
Description:
O00311[58-574]
Residue:
517
Sequence:
FKIEDKIGEGTFSSVYLATAQLQVGPEEKIALKHLIPTSHPIRIAAELQCLTVAGGQDNVMGVKYCFRKNDHVVIAMPYLEHESFLDILNSLSFQEVREYMLNLFKALKRIHQFGIVHRDVKPSNFLYNRRLKKYALVDFGLAQGTHDTKIELLKFVQSEAQQERCSQNKSHIITGNKIPLSGPVPKELDQQSTTKASVKRPYTNAQIQIKQGKDGKEGSVGLSVQRSVFGERNFNIHSSISHESPAVKLMKQSKTVDVLSRKLATKKKAISTKVMNSAVMRKTASSCPASLTCDCYATDKVCSICLSRRQQVAPRAGTPGFRAPEVLTKCPNQTTAIDMWSAGVIFLSLLSGRYPFYKASDDLTALAQIMTIRGSRETIQAAKTFGKSILCSKEVPAQDLRKLCERLRGMDSSTPKLTSDIQGHASHQPAISEKTDHKASCLVQTPPGQYSGNSFKKGDSNSCEHCFDEYNTNLEGWNEVPDEAYDLLDKLLDLNPASRITAEEALLHPFFKDMSL
  
Component 2
Name:
Protein DBF4 homolog A
Synonyms:
ASK | Activator of S phase kinase | Chiffon homolog A | DBF4 | DBF4-type zinc finger-containing protein 1 | DBF4A | DBF4A_HUMAN | Protein DBF4 homolog A | ZDBF1
Type:
Regulatory subunit
Mol. Mass.:
76869.65
Organism:
Homo sapiens (Human)
Description:
n/a
Residue:
674
Sequence:
MNSGAMRIHSKGHFQGGIQVKNEKNRPSLKSLKTDNRPEKSKCKPLWGKVFYLDLPSVTISEKLQKDIKDLGGRVEEFLSKDISYLISNKKEAKFAQTLGRISPVPSPESAYTAETTSPHPSHDGSSFKSPDTVCLSRGKLLVEKAIKDHDFIPSNSILSNALSWGVKILHIDDIRYYIEQKKKELYLLKKSSTSVRDGGKRVGSGAQKTRTGRLKKPFVKVEDMSQLYRPFYLQLTNMPFINYSIQKPCSPFDVDKPSSMQKQTQVKLRIQTDGDKYGGTSIQLQLKEKKKKGYCECCLQKYEDLETHLLSEQHRNFAQSNQYQVVDDIVSKLVFDFVEYEKDTPKKKRIKYSVGSLSPVSASVLKKTEQKEKVELQHISQKDCQEDDTTVKEQNFLYKETQETEKKLLFISEPIPHPSNELRGLNEKMSNKCSMLSTAEDDIRQNFTQLPLHKNKQECILDISEHTLSENDLEELRVDHYKCNIQASVHVSDFSTDNSGSQPKQKSDTVLFPAKDLKEKDLHSIFTHDSGLITINSSQEHLTVQAKAPFHTPPEEPNECDFKNMDSLPSGKIHRKVKIILGRNRKENLEPNAEFDKRTEFITQEENRICSSPVQSLLDLFQTSEEKSEFLGFTSYTEKSGICNVLDIWEEENSDNLLTAFFSSPSTSTFTGF
  
Inhibitor
Name:
BDBM27433
Synonyms:
16-methyl-8-thia-3,5,10-triazatetracyclo[7.7.0.0^{2,7}.0^{11,15}]hexadeca-1(16),2(7),3,5,9,11(15)-hexaen-6-amine | pyrido-thieno-pyrimidine, 6g
Type:
Small organic molecule
Emp. Form.:
C13H12N4S
Mol. Mass.:
256.326
SMILES:
Cc1c2CCCc2nc2sc3c(N)ncnc3c12
Structure:
Search PDB for entries with ligand similarity:
Substrate
Name:
His6-tagged MCM2
Synonyms:
BM28 | CCNL1 | CDCL1 | DNA replication licensing factor MCM2 | KIAA0030 | MCM2 | MCM2_HUMAN | Minichromosome maintenance protein 2 homolog | Nuclear protein BM28
Type:
Other Protein Type
Mol. Mass.:
52558.30
Organism:
Homo sapiens (Human)
Description:
P49736[1-467]
Residue:
467
Sequence:
MAESSESFTMASSPAQRRRGNDPLTSSPGRSSRRTDALTSSPGRDLPPFEDESEGLLGTEGPLEEEEDGEELIGDGMERDYRAIPELDAYEAEGLALDDEDVEELTASQREAAERAMRQRDREAGRGLGRMRRGLLYDSDEEDEERPARKRRQVERATEDGEEDEEMIESIENLEDLKGHSVREWVSMAGPRLEIHHRFKNFLRTHVDSHGHNVFKERISDMCKENRESLVVNYEDLAAREHVLAYFLPEAPAELLQIFDEAALEVVLAMYPKYDRITNHIHVRISHLPLVEELRSLRQLHLNQLIRTSGVVTSCTGVLPQLSMVKYNCNKCNFVLGPFCQSQNQEVKPGSCPECQSAGPFEVNMEETIYQNYQRIRIQESPGKVAAGRLPRSKDAILLADLVDSCKPGDEIELTGIYHNNYDGSLNTANGFPVFATVILANHVAKKDNKVAVGELTDEDVKMITSL