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Target
RAF proto-oncogene serine/threonine-protein kinase [1-324]
Ligand
BDBM202843
Substrate
MEK1 (K97R)
Meas. Tech.
Raf Activity Assay
pH
7.5±n/a
Temperature
298.15±n/a K
IC50
0.62±n/a nM
Comments
extracted
Citation
 Aversa, RJ; Barsanti, PA; Burger, M; Dillon, MP; Dipesa, A; Hu, C; Lou, Y; Nishiguchi, G; Pan, Y; Polyakov, V; Ramurthy, S; Rico, A; Setti, L; Smith, A; Subramanian, S; Taft, B; Tanner, H; Wan, L; Yusuff, N Biaryl amide compounds as kinase inhibitors US Patent  US9694016 Publication Date 7/4/2017 
Target
Name:
RAF proto-oncogene serine/threonine-protein kinase [1-324]
Synonyms:
C-Raf TR (aa 1-324) | Proto-oncogene C-Raf (C-Raf TR) | RAF | RAF1 | RAF1_HUMAN
Type:
n/a
Mol. Mass.:
36996.69
Organism:
Homo sapiens (Human)
Description:
P04049 (1-324)
Residue:
324
Sequence:
QEKNKIRPRGQRDSSEEWEIEASEVMLSTRIGSGSFGTVYKGKWHGDVAVKILKVVDPTPEQFQAFRNEVAVLRKTRHVNILLFMGYMTKDNLAIVTQWCEGSSLYKHLHVQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKSRWSGSQQVEQPTGSVLWMAPEVIRMQDNNPFSFQSDVYSYGIVLYELMTGELPYSHINNRDQIIFMVGRGYASPDLSKLYKNCPKAMKRLVADCVKKVKEERPLFPQILSSIELLQHSLPKINRSASEPSLHRAAHTEDINACTLTTSPRLPVF
  
Inhibitor
Name:
BDBM202843
Synonyms:
US10245267, Example 196 | US10709712, Example 196 | US9242969, 196 | US9694016, 196
Type:
Small organic molecule
Emp. Form.:
C25H24F3N3O3
Mol. Mass.:
471.4716
SMILES:
COc1cc(nc(c1)-c1cc(NC(=O)c2cccc(c2)C(F)(F)F)ccc1C)N1CCOCC1
Structure:
Search PDB for entries with ligand similarity:
Substrate
Name:
Dual specificity mitogen-activated protein kinase kinase 1 [K97R]
Synonyms:
Dual specificity mitogen-activated protein kinase kinase 1 (MEK1)(K97R) | MAP2K1 | MEK1 | MEK1 (K97R) | MP2K1_HUMAN | PRKMK1
Type:
n/a
Mol. Mass.:
43467.04
Organism:
Homo sapiens (Human)
Description:
Biotinylated full length MEK1 with an inactivating K97R ATP binding site mutation.
Residue:
393
Sequence:
MPKKKPTPIQLNPAPDGSAVNGTSSAETNLEALQKKLEELELDEQQRKRLEAFLTQKQKVGELKDDDFEKISELGAGNGGVVFKVSHKPSGLVMARRLIHLEIKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKKAGRIPEQILGKVSIAVIKGLTYLREKHKIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDSMANSFVGTRSYMSPERLQGTHYSVQSDIWSMGLSLVEMAVGRYPIPPPDAKELELMFGCQVEGDAAETPPRPRTPGRPLSSYGMDSRPPMAIFELLDYIVNEPPPKLPSGVFSLEFQDFVNKCLIKNPAERADLKQLMVHAFIKRSDAEEVDFAGWLCSTIGLNQPSTPTHAAGV