| Reaction Details |
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 | Report a problem with these data |
| Target | Runt-Related Transcription Factor 1 Isoform AML1C |
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| Ligand | BDBM29643 |
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| Substrate/Competitor | Venus-CBFbeta-SMMHC |
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| Meas. Tech. | Confirmation of compounds inhibiting the binding between the RUNX1 Runt domain and CBFb-SMMHC via a time resolved fluorescence resonance energy transfer (TR-FRET) assay. |
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| pH | 7.4±n/a |
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| Temperature | 296.15±n/a K |
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| IC50 | 17777±n/a nM |
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| Citation |  PubChem, PC Confirmation of compounds inhibiting the binding between the RUNX1 Runt domain and CBFbeta-SMMHC via a time resolved fluorescence resonance energy transfer (TR-FRET) assay. PubChem Bioassay(2009)[AID] |
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| More Info.: | Get all data from this article, Solution Info, Assay Method |
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| Runt-Related Transcription Factor 1 Isoform AML1C |
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| Name: | Runt-Related Transcription Factor 1 Isoform AML1C |
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| Synonyms: | Acute myeloid leukemia 1 protein | CBF-alpha-2 | Core-binding factor subunit alpha-2 | Oncogene AML-1 | Polyomavirus enhancer-binding protein 2 alpha B subunit |
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| Type: | Subunit of heterodimeric transcription factor |
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| Mol. Mass.: | 48750.25 |
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| Organism: | Homo sapiens (Human) |
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| Description: | Q01196 |
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| Residue: | 453 |
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| Sequence: | MRIPVDASTSRRFTPPSTALSPGKMSEALPLGAPDAGAALAGKLRSGDRSMVEVLADHPG
ELVRTDSPNFLCSVLPTHWRCNKTLPIAFKVVALGDVPDGTLVTVMAGNDENYSAELRNA
TAAMKNQVARFNDLRFVGRSGRGKSFTLTITVFTNPPQVATYHRAIKITVDGPREPRRHR
QKLDDQTKPGSLSFSERLSELEQLRRTAMRVSPHHPAPTPNPRASLNHSTAFNPQPQSQM
QDTRQIQPSPPWSYDQSYQYLGSIASPSVHPATPISPGRASGMTTLSAELSSRLSTAPDL
TAFSDPRQFPALPSISDPRMHYPGAFTYSPTPVTSGIGIGMSAMGSATRYHTYLPPPYPG
SSQAQGGPFQASSPSYHLYYGASAGSYQFSMVGGERSPPRILPPCTNASTGSALLNPSLP
NQSDVVEAEGSHSNSPTNMAPSARLEEAVWRPY
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| BDBM29643 |
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| Name | BDBM29643 |
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| Synonyms: | (6aS)-6-methyl-5,6,6a,7-tetrahydro-4H-dibenzo[de,g]quinoline-10,11-diol;hydrochloride | (S)-6-methyl-5,6,6a,7-tetrahydro-4H-dibenzo[de,g]quinoline-10,11-diol | Apomorphine,(+) | CHEMBL416288 | MLS000028867 | S(+)-10,11-DIHYDROXYAPORPHINE HYDROCHLORIDE | SMR000058671 | cid_6852389 |
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| Type | Small organic molecule |
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| Emp. Form. | C17H17NO2 |
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| Mol. Mass. | 267.3224 |
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| SMILES | CN1CCc2cccc-3c2[C@@H]1Cc1ccc(O)c(O)c-31 |
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| Structure |  |
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| Venus-CBFbeta-SMMHC |
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| Name: | Venus-CBFbeta-SMMHC |
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| Synonyms: | CBFbeta | Core-binding factor subunit beta isoform 2 | Venus-core-binding factor subunit beta-smooth muscle myosin heavy chain |
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| Type: | Fluorescent fusion protein |
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| Mol. Mass.: | 21988.19 |
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| Organism: | Homo sapiens (Human) |
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| Description: | Venus-CBFbeta-SMMHC was provided by Dr. John Bushweller (University of Virginia Charlottesville, Charlottesville VA). Only the amino acid sequence of core-binding factor subunit beta (CBFbeta) isoform 2 is shown here. |
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| Residue: | 187 |
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| Sequence: | MPRVVPDQRSKFENEEFFRKLSRECEIKYTGFRDRPHEERQARFQNACRDGRSEIAFVAT
GTNLSLQFFPASWQGEQRQTPSREYVDLEREAGKVYLKAPMILNGVCVIWKGWIDLQRLD
GMGCLEFDEERAQQEDALAQQAFEEARRRTREFEDRDRSHREEMEARRQQDPSPGSNLGG
GDDLKLR
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