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Target
E3 ubiquitin-protein ligase Mdm2
Ligand
BDBM31228
Substrate
biotinylated IP3 peptide
Meas. Tech.
MDM2-p53 Interaction Using a 96-Well Plate Binding Assay (ELISA)
pH
7.4±n/a
Temperature
277.15±n/a K
IC50
90000±39000 nM
Citation
 Hardcastle, IR; Ahmed, SU; Atkins, H; Farnie, G; Golding, BT; Griffin, RJ; Guyenne, S; Hutton, C; Källblad, P; Kemp, SJ; Kitching, MS; Newell, DR; Norbedo, S; Northen, JS; Reid, RJ; Saravanan, K; Willems, HM; Lunec, J Small-molecule inhibitors of the MDM2-p53 protein-protein interaction based on an isoindolinone scaffold. J Med Chem 49:6209-21 (2006) [PubMed]  Article 
Target
Name:
E3 ubiquitin-protein ligase Mdm2
Synonyms:
Double minute 2 protein | Double minute 2 protein (HDM2) | E3 ubiquitin-protein ligase Mdm2 (p53-binding protein Mdm2) | Hdm2 | Human Double Minute 2 (HDM2) | MDM2 | MDM2-MDMX | MDM2_HUMAN | p53-Binding Protein MDM2 | p53-binding protein
Type:
Oncoprotein
Mol. Mass.:
55196.54
Organism:
Homo sapiens (Human)
Description:
Q00987
Residue:
491
Sequence:
MCNTNMSVPTDGAVTTSQIPASEQETLVRPKPLLLKLLKSVGAQKDTYTMKEVLFYLGQYIMTKRLYDEKQQHIVYCSNDLLGDLFGVPSFSVKEHRKIYTMIYRNLVVVNQQESSDSGTSVSENRCHLEGGSDQKDLVQELQEEKPSSSHLVSRPSTSSRRRAISETEENSDELSGERQRKRHKSDSISLSFDESLALCVIREICCERSSSSESTGTPSNPDLDAGVSEHSGDWLDQDSVSDQFSVEFEVESLDSEDYSLSEEGQELSDEDDEVYQVTVYQAGESDTDSFEEDPEISLADYWKCTSCNEMNPPLPSHCNRCWALRENWLPEDKGKDKGEISEKAKLENSTQAEEGFDVPDCKKTIVNDSRESCVEENDDKITQASQSQESEDYSQPSTSSSIIYSSQEDVKEFEREETQDKEESVESSLPLNAIEPCVICQGRPKNGCIVHGKTGHLMACFTCAKKLKKRNKPCPVCRQPIQMIVLTYFP
  
Inhibitor
Name:
BDBM31228
Synonyms:
CHEMBL182052 | isoindolinone scaffold, 14
Type:
Small organic molecule
Emp. Form.:
C27H27NO5
Mol. Mass.:
445.507
SMILES:
CCCOC1(N(C(Cc2ccc(O)cc2)C(=O)OC)C(=O)c2ccccc12)c1ccccc1
Structure:
Search PDB for entries with ligand similarity:
Substrate
Name:
biotinylated IP3 peptide
Synonyms:
b-IP3
Type:
Peptide
Mol. Mass.:
4116.56
Organism:
n/a
Description:
n/a
Residue:
37
Sequence:
ACMETPRARGPHEMETASPTYRTRPGLGLYLEASNNH