Reaction Details Report a problem with these data
Target
Collagenase 3
Ligand
BDBM32410
Substrate
5-FAM/QXL 520 Peptide Substrate
Meas. Tech.
Fluorescence Resonance Energy Transfer (FRET) Assay
pH
7.5±n/a
Temperature
310.15±n/a K
IC50
220±n/a nM
Citation
 Heim-Riether, A; Taylor, SJ; Liang, S; Gao, DA; Xiong, Z; Michael August, E; Collins, BK; Farmer, BT; Haverty, K; Hill-Drzewi, M; Junker, HD; Mariana Margarit, S; Moss, N; Neumann, T; Proudfoot, JR; Keenan, LS; Sekul, R; Zhang, Q; Li, J; Farrow, NA Improving potency and selectivity of a new class of non-Zn-chelating MMP-13 inhibitors. Bioorg Med Chem Lett 19:5321-4 (2009) [PubMed]  Article 
Target
Name:
Collagenase 3
Synonyms:
MMP-13 | MMP13 | MMP13_HUMAN | Matrix metalloproteinase-13 | Matrix metalloproteinase-13 (MMP-13) | Matrix metalloproteinase-13 (MMP13)
Type:
Enzyme
Mol. Mass.:
53808.06
Organism:
Homo sapiens (Human)
Description:
P45452
Residue:
471
Sequence:
MHPGVLAAFLFLSWTHCRALPLPSGGDEDDLSEEDLQFAERYLRSYYHPTNLAGILKENAASSMTERLREMQSFFGLEVTGKLDDNTLDVMKKPRCGVPDVGEYNVFPRTLKWSKMNLTYRIVNYTPDMTHSEVEKAFKKAFKVWSDVTPLNFTRLHDGIADIMISFGIKEHGDFYPFDGPSGLLAHAFPPGPNYGGDAHFDDDETWTSSSKGYNLFLVAAHEFGHSLGLDHSKDPGALMFPIYTYTGKSHFMLPDDDVQGIQSLYGPGDEDPNPKHPKTPDKCDPSLSLDAITSLRGETMIFKDRFFWRLHPQQVDAELFLTKSFWPELPNRIDAAYEHPSHDLIFIFRGRKFWALNGYDILEGYPKKISELGLPKEVKKISAAVHFEDTGKTLLFSGNQVWRYDDTNHIMDKDYPRLIEEDFPGIGDKVDAVYEKNGYIYFFNGPIQFEYSIWSNRIVRVMPANSILWC
  
Inhibitor
Name:
BDBM32410
Synonyms:
furan-2-carboxamide deriv., 19
Type:
Small organic molecule
Emp. Form.:
C21H26N2O4
Mol. Mass.:
370.4421
SMILES:
CNC(=O)[C@@H](NC(=O)c1ccc(o1)-c1ccc(OC)cc1)C1CCCCC1 |r|
Structure:
Search PDB for entries with ligand similarity:
Substrate
Name:
5-FAM/QXL 520 Peptide Substrate
Synonyms:
n/a
Type:
FRET Peptide
Mol. Mass.:
1848.68
Organism:
n/a
Description:
EnzoLyte520 Generic MMP Assay Kit (AnaSpec Inc.).
Residue:
15
Sequence:
QLPLAYWARKFAMNH