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Target
Isoform Alpha-1 of Mitogen-activated protein kinase 10 (Alpha-1) 9-402]
Ligand
BDBM32461
Substrate
biotinylated-ATF2
Meas. Tech.
Homogeneous Time-resolved Fluorescence Assay
pH
7±n/a
Temperature
295.15±n/a K
IC50
960±n/a nM
Citation
 Shin, Y; Chen, W; Habel, J; Duckett, D; Ling, YY; Koenig, M; He, Y; Vojkovsky, T; LoGrasso, P; Kamenecka, TM Synthesis and SAR of piperazine amides as novel c-jun N-terminal kinase (JNK) inhibitors. Bioorg Med Chem Lett 19:3344-7 (2009) [PubMed]  Article 
Target
Name:
Isoform Alpha-1 of Mitogen-activated protein kinase 10 (Alpha-1) 9-402]
Synonyms:
JNK3 | JNK3A | MAP kinase p49 3F12 | MAPK10 | MK10_HUMAN | Mitogen-Activated Protein Kinase 10 (JNK3) | Mitogen-activated protein kinase 10 (Alpha-1) [39-402] | PRKM10 | SAPK1B | Stress-activated protein kinase JNK3
Type:
Serine/threonine-protein kinase
Mol. Mass.:
42009.30
Organism:
Homo sapiens (Human)
Description:
P53779-2[39-402]
Residue:
364
Sequence:
MSKSKVDNQFYSVEVGDSTFTVLKRYQNLKPIGSGAQGIVCAAYDAVLDRNVAIKKLSRPFQNQTHAKRAYRELVLMKCVNHKNIISLLNVFTPQKTLEEFQDVYLVMELMDANLCQVIQMELDHERMSYLLYQMLCGIKHLHSAGIIHRDLKPSNIVVKSDCTLKILDFGLARTAGTSFMMTPYVVTRYYRAPEVILGMGYKENVDIWSVGCIMGEMVRHKILFPGRDYIDQWNKVIEQLGTPCPEFMKKLQPTVRNYVENRPKYAGLTFPKLFPDSLFPADSEHNKLKASQARDLLSKMLVIDPAKRISVDDALQHPYINVWYDPAEVEAPPPQIYDKQLDEREHTIEEWKELIYKEVMNSE
  
Inhibitor
Name:
BDBM32461
Synonyms:
piperazine amide, 4h
Type:
Small organic molecule
Emp. Form.:
C18H19BrClN3O2
Mol. Mass.:
424.719
SMILES:
Clc1cccc(NC(=O)c2ccc(Br)o2)c1N1CCN(CC1)C1CC1
Structure:
Search PDB for entries with ligand similarity:
Substrate
Name:
biotinylated-ATF2
Synonyms:
Activating transcription factor 2 | Cyclic AMP-dependent transcription factor ATF-2 | cAMP response element-binding protein CRE-BP1
Type:
Other Protein Type
Mol. Mass.:
13150.37
Organism:
Homo sapiens (Human)
Description:
ATF-2 (aa 1-115) was expressed and purified from E. coli.
Residue:
115
Sequence:
MKFKLHVNSARQYKDLWNMSDDKPFLCTAPGCGQRFTNEDHLAVHKHKHEMTLKFGPARNDSVIVADQTPTPTRFLKNCEEVGLFNELASPFENEFKKASEDDIKKMPLDLSPLA