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Target
Gag-Pol polyprotein [600-1028,C879S]/[600-1151,C879S]
Ligand
BDBM33959
Substrate
RNA/DNA hybrid
Meas. Tech.
Assay of RT RNH Activity
pH
8±n/a
Temperature
310.15±n/a K
IC50
5000±n/a nM
Citation
 Himmel, DM; Sarafianos, SG; Dharmasena, S; Hossain, MM; McCoy-Simandle, K; Ilina, T; Clark, AD; Knight, JL; Julias, JG; Clark, PK; Krogh-Jespersen, K; Levy, RM; Hughes, SH; Parniak, MA; Arnold, E HIV-1 reverse transcriptase structure with RNase H inhibitor dihydroxy benzoyl naphthyl hydrazone bound at a novel site. ACS Chem Biol 1:702-12 (2006) [PubMed]  Article 
Target
Name:
Gag-Pol polyprotein [600-1028,C879S]/[600-1151,C879S]
Synonyms:
HIV-1 Reverse Transcriptase RNase H
Type:
Protein Complex
Mol. Mass.:
n/a
Description:
n/a
Components:
This complex has 2 components.
Component 1
Name:
Gag-Pol polyprotein [600-1151,C879S]
Synonyms:
HIV-1 Reverse Transcriptase RNase H Chain A | POL_HV1B1 | gag-pol
Type:
Enzyme Subunit
Mol. Mass.:
63584.38
Organism:
Human immunodeficiency virus type 1
Description:
P03366[600-1151,C879S]
Residue:
552
Sequence:
PISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKKKKSVTVLDVGDAYFSVPLDEDFRKYTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQSSMTKILEPFKKQNPDIVIYQYMDDLYVGSDLEIGQHRTKIEELRQHLLRWGLTTPDKKHQKEPPFLWMGYELHPDKWTVQPIVLPEKDSWTVNDIQKLVGKLNWASQIYPGIKVRQLSKLLRGTKALTEVIPLTEEAELELAENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMRGAHTNDVKQLTEAVQKITTESIVIWGKTPKFKLPIQKETWETWWTEYWQATWIPEWEFVNTPPLVKLWYQLEKEPIVGAETFYVDGAANRETKLGKAGYVTNKGRQKVVPLTNTTNQKTELQAIYLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVNQIIEQLIKKEKVYLAWVPAHKGIGGNEQVDKLV
  
Component 2
Name:
Gag-Pol polyprotein [600-1028,C879S]
Synonyms:
HIV-1 Reverse Transcriptase RNase H Chain B | POL_HV1B1 | gag-pol
Type:
Enzyme Subunit
Mol. Mass.:
50093.74
Organism:
Human immunodeficiency virus type 1
Description:
P03366[600-1028,C879S]
Residue:
429
Sequence:
PISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKKKKSVTVLDVGDAYFSVPLDEDFRKYTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQSSMTKILEPFKKQNPDIVIYQYMDDLYVGSDLEIGQHRTKIEELRQHLLRWGLTTPDKKHQKEPPFLWMGYELHPDKWTVQPIVLPEKDSWTVNDIQKLVGKLNWASQIYPGIKVRQLSKLLRGTKALTEVIPLTEEAELELAENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMRGAHTNDVKQLTEAVQKITTESIVIWGKTPKFKLPIQKETWETWWTEYWQATWIPEWEFVNTPPLVKLWYQL
  
Inhibitor
Name:
BDBM33959
Synonyms:
dihydroxy benzoyl naphthyl hydrazone (DHBNH) analogue, 6
Type:
Small organic molecule
Emp. Form.:
C25H20N2O2
Mol. Mass.:
380.4385
SMILES:
COc1ccc2ccccc2c1\C=N\NC(=O)c1ccc(cc1)-c1ccccc1
Structure:
Search PDB for entries with ligand similarity:
Substrate
Name:
RNA/DNA hybrid
Synonyms:
n/a
Type:
fluorescein-labeled RNA annealed to DNA
Mol. Mass.:
2134.40
Organism:
n/a
Description:
It is a 18-nucleotide 3-fluorescein-labeled RNA annealed to a complementary 18-nucleotide 5-Dabcyl-modified DNA.
Residue:
23
Sequence:
GACGAGCCGGGAGCFLRESCEIN