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Target
Gag-Pol polyprotein [600-1028,L699I,K702N,C879S]/[600-1151,L699I,K702N,C879S]
Ligand
BDBM33953
Substrate
RNA/DNA hybrid
Meas. Tech.
Assay of RT RNH Activity
IC50
700±n/a nM
Citation
 Himmel, DM; Sarafianos, SG; Dharmasena, S; Hossain, MM; McCoy-Simandle, K; Ilina, T; Clark, AD; Knight, JL; Julias, JG; Clark, PK; Krogh-Jespersen, K; Levy, RM; Hughes, SH; Parniak, MA; Arnold, E HIV-1 reverse transcriptase structure with RNase H inhibitor dihydroxy benzoyl naphthyl hydrazone bound at a novel site. ACS Chem Biol 1:702-12 (2006) [PubMed]  Article 
Target
Name:
Gag-Pol polyprotein [600-1028,L699I,K702N,C879S]/[600-1151,L699I,K702N,C879S]
Synonyms:
HIV-1 Reverse Transcriptase RNase H Mutant (L100I/K103N)
Type:
Protein Complex
Mol. Mass.:
n/a
Description:
n/a
Components:
This complex has 2 components.
Component 1
Name:
Gag-Pol polyprotein [600-1151,L699I,K702N,C879S]
Synonyms:
HIV-1 Reverse Transcriptase RNase H Mutant (L100I/K103N) Chain A | POL_HV1B1 | gag-pol
Type:
Enzyme Subunit
Mol. Mass.:
63569.30
Organism:
Human immunodeficiency virus type 1
Description:
P03366[600-1151,L699I,K702N,C879S]
Residue:
552
Sequence:
PISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPHPAGIKKNKSVTVLDVGDAYFSVPLDEDFRKYTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQSSMTKILEPFKKQNPDIVIYQYMDDLYVGSDLEIGQHRTKIEELRQHLLRWGLTTPDKKHQKEPPFLWMGYELHPDKWTVQPIVLPEKDSWTVNDIQKLVGKLNWASQIYPGIKVRQLSKLLRGTKALTEVIPLTEEAELELAENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMRGAHTNDVKQLTEAVQKITTESIVIWGKTPKFKLPIQKETWETWWTEYWQATWIPEWEFVNTPPLVKLWYQLEKEPIVGAETFYVDGAANRETKLGKAGYVTNKGRQKVVPLTNTTNQKTELQAIYLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVNQIIEQLIKKEKVYLAWVPAHKGIGGNEQVDKLV
  
Component 2
Name:
Gag-Pol polyprotein [600-1028,L699I,K702N,C879S]
Synonyms:
HIV-1 Reverse Transcriptase RNase H Mutant (L100I/K103N) Chain B | POL_HV1B1 | gag-pol
Type:
Enzyme Subunit
Mol. Mass.:
50078.66
Organism:
Human immunodeficiency virus type 1
Description:
P03366[600-1028,L699I,K702N,C879S]
Residue:
429
Sequence:
PISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPHPAGIKKNKSVTVLDVGDAYFSVPLDEDFRKYTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQSSMTKILEPFKKQNPDIVIYQYMDDLYVGSDLEIGQHRTKIEELRQHLLRWGLTTPDKKHQKEPPFLWMGYELHPDKWTVQPIVLPEKDSWTVNDIQKLVGKLNWASQIYPGIKVRQLSKLLRGTKALTEVIPLTEEAELELAENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMRGAHTNDVKQLTEAVQKITTESIVIWGKTPKFKLPIQKETWETWWTEYWQATWIPEWEFVNTPPLVKLWYQL
  
Inhibitor
Name:
BDBM33953
Synonyms:
dihydroxy benzoyl naphthyl hydrazone (DHBNH)
Type:
Small organic molecule
Emp. Form.:
C19H16N2O4
Mol. Mass.:
336.3413
SMILES:
COc1ccc2ccccc2c1\C=N\NC(=O)c1ccc(O)c(O)c1
Structure:
Search PDB for entries with ligand similarity:
Substrate
Name:
RNA/DNA hybrid
Synonyms:
n/a
Type:
fluorescein-labeled RNA annealed to DNA
Mol. Mass.:
2134.40
Organism:
n/a
Description:
It is a 18-nucleotide 3-fluorescein-labeled RNA annealed to a complementary 18-nucleotide 5-Dabcyl-modified DNA.
Residue:
23
Sequence:
GACGAGCCGGGAGCFLRESCEIN