Reaction Details Report a problem with these data
Target
E3 ubiquitin-protein ligase Mdm2
Ligand
BDBM35075
Substrate
p53 Peptide
Meas. Tech.
Fluorescence Anisotropy Competition Binding Assay
pH
7.6±n/a
Temperature
296.15±n/a K
IC50
8200±300 nM
Citation
 Hayashi, R; Wang, D; Hara, T; Iera, JA; Durell, SR; Appella, DH N-acylpolyamine inhibitors of HDM2 and HDMX binding to p53. Bioorg Med Chem 17:7884-93 (2009) [PubMed]  Article 
Target
Name:
E3 ubiquitin-protein ligase Mdm2
Synonyms:
Double minute 2 protein | Double minute 2 protein (HDM2) | E3 ubiquitin-protein ligase Mdm2 (p53-binding protein Mdm2) | Hdm2 | Human Double Minute 2 (HDM2) | MDM2 | MDM2-MDMX | MDM2_HUMAN | p53-Binding Protein MDM2 | p53-binding protein
Type:
Oncoprotein
Mol. Mass.:
55196.54
Organism:
Homo sapiens (Human)
Description:
Q00987
Residue:
491
Sequence:
MCNTNMSVPTDGAVTTSQIPASEQETLVRPKPLLLKLLKSVGAQKDTYTMKEVLFYLGQYIMTKRLYDEKQQHIVYCSNDLLGDLFGVPSFSVKEHRKIYTMIYRNLVVVNQQESSDSGTSVSENRCHLEGGSDQKDLVQELQEEKPSSSHLVSRPSTSSRRRAISETEENSDELSGERQRKRHKSDSISLSFDESLALCVIREICCERSSSSESTGTPSNPDLDAGVSEHSGDWLDQDSVSDQFSVEFEVESLDSEDYSLSEEGQELSDEDDEVYQVTVYQAGESDTDSFEEDPEISLADYWKCTSCNEMNPPLPSHCNRCWALRENWLPEDKGKDKGEISEKAKLENSTQAEEGFDVPDCKKTIVNDSRESCVEENDDKITQASQSQESEDYSQPSTSSSIIYSSQEDVKEFEREETQDKEESVESSLPLNAIEPCVICQGRPKNGCIVHGKTGHLMACFTCAKKLKKRNKPCPVCRQPIQMIVLTYFP
  
Inhibitor
Name:
BDBM35075
Synonyms:
N-acylpolyamine (NAPA) scaffold, 22
Type:
Small organic molecule
Emp. Form.:
C53H82Br2N10O9
Mol. Mass.:
1163.088
SMILES:
NCCCCC(CN(C(CCC(O)=O)CN(CCC(N)=O)C(=O)NCCCc1ccc(Br)cc1)C(=O)NCCc1ccc(Br)cc1)N(CC(CCC(O)=O)NC(=O)[C@@H]1CCCN1)C(=O)NCCCC1CCCCC1 |r|
Structure:
Search PDB for entries with ligand similarity:
Substrate
Name:
Cellular tumor antigen p53 (aa 15-29)
Synonyms:
P53 | P53_TUPBE | TP53 | p53 (aa 15-29)
Type:
fluorescein-conjugated peptide
Mol. Mass.:
1804.95
Organism:
Tupaia belangeri (Common tree shrew) (Tupaia glis belangeri)
Description:
Q9TTA1 aa 15-29
Residue:
15
Sequence:
SQETFSDLWKLLPEN