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Target
Genome polyprotein
Ligand
BDBM35553
Substrate
poly(A)/oligo(U)18
Meas. Tech.
HCV RNA-Dependent RNA Polymerase Assay (IC50)
IC50
540±n/a nM
Citation
 Ontoria, JM; Rydberg, EH; Di Marco, S; Tomei, L; Attenni, B; Malancona, S; Martin Hernando, JI; Gennari, N; Koch, U; Narjes, F; Rowley, M; Summa, V; Carroll, SS; Olsen, DB; De Francesco, R; Altamura, S; Migliaccio, G; Carfì, A Identification and Biological Evaluation of a Series of 1H-Benzo[de]isoquinoline-1,3(2H)-diones as Hepatitis C Virus NS5B Polymerase Inhibitors. J Med Chem 52:5217-27 (2009) [PubMed]  Article 
Target
Name:
Genome polyprotein
Synonyms:
HCV NS5B Polymerase | POLG_HCVED | RNA-directed RNA polymerase | p68
Type:
Enzyme
Mol. Mass.:
327656.24
Organism:
Hepatitis C virus genotype 4a (isolate ED43) (HCV)
Description:
O39929
Residue:
3008
Sequence:
MSTNPKPQRKTKRNTNRRPMDVKFPGGGQIVGGVYLLPRRGPRLGVRATRKTSERSQPRGRRQPIPKARRPEGRSWAQPGYPWPLYGNEGCGWAGWLLSPRGSRPSWGPNDPRGRSRNLGKVIDTLTCGFADLMGYIPLVGAPVGSVARALAHGVRALEDGINYATGNLPGCSFSIFLLALLSCLTVPASAVNYRNVSGIYHVTNDCPNSSIVYEADHHIMHLPGCVPCVREGNQSRCWVALTPTVAAPYIGAPLESLRSHVDLMVGAATVCSGLYIGDLCGGLFLVGQMFSFRPRRHWTTQDCNCSIYTGHITGHRMAWDMMMNWSPTTTLVLAQVMRIPTTLVDLLSGGHWGVLVGVAYFSMQANWAKVILVLFLFAGVDAETHVSGAAVGRSTAGLANLFSSGSKQNLQLINSNGSWHINRTALNCNDSLNTGFLASLFYTHKFNSSGCSERLACCKSLDSYGQGWGPLGVANISGSSDDRPYCWHYAPRPCGIVPASSVCGPVYCFTPSPVVVGTTDHVGVPTYTWGENETDVFLLNSTRPPHGAWFGCVWMNSTGFTKTCGAPPCEVNTNNGTWHCPTDCFRKHPETTYAKCGSGPWITPRCLIDYPYRLWHFPCTANFSVFNIRTFVGGIEHRMQAACNWTRGEVCGLEHRDRVELSPLLLTTTAWQILPCSFTTLPALSTGLIHLHQNIVDVQYLYGVGSAVVSWALKWEYVVLAFLLLADARVSAYLWMMFMVSQVEAALSNLININAASAAGAQGFWYAILFICIVWHVKGRFPAAAAYAACGLWPCFLLLLMLPERAYAYDQEVAGSLGGAIVVMLTILTLSPHYKLWLARGLWWIQYFIARTEAVLHVYIPSFNVRGPRDSVIVLAVLVCPDLVFDITKYLLAILGPLHILQASLLRIPYFVRAQALVKICSLLRGVVYGKYFQMVVLKSRGLTGTYIYDHLTPMSDWPPYGLRDLAVALEPVVFTPMEKKVIVWGADTAACGDIIRGLPVSARLGNEILLGPADTETSKGWRLLAPITAYAQQTRGLFSTIVTSLTGRDTNENCGEVQVLSTATQSFLGTAVNGVMWTVYHGAGAKTISGPKGPVNQMYTNVDQDLVGWPAPPGVRSLAPCTCGSADLYLVTRHADVIPVRRRGDTRGALLSPRPISILKGSSGGPLLCPMGHRAGIFRAAVCTRGVAKAVDFVPVESLETTMRSPVFTDNSTPPAVPQTYQVAHLHAPTGSGKSTKVPAAHAAQGYKVLVLNPSVAATLGFGVYMSKAYGIDPNIRSGVRTITTGAPITYSTYGKFLADGGCSGGAYDIIICDECYSTDSTTILGIGTVLDQAETAGVRLTVLATATPPGSVTTPHSNIEEVALPTTGEIPFYGKAIPLELIKGGRHLIFCHSKKKCDELARQLTSLGLNAVAYYRGLDVSVIPTSGDVVVCATDALMTGFTGDFDSVIDCNTSVIQTVDFSLDPTFSIEITTVPQDAVSRSQRRGRTGRGRLGTYRYVTPGERPSGMFDTAELCECYDAGCAWYELTPAETTTRLKAYFDTPGLPVCQDHLEFWESVFTGLTHIDGHFLSQTKQSGENFPYLVAYQATVSAKVWLAPPSWDTMWKCLIRLKPTLHGPTPLLYRLGSVQNEVVLTHPITKYIMACMSADLEVVTSTWVLVGGVLAALAAYCLSVGSVVIVGRVVLSGQPAVIPDREVLYQQFDEMEECSKHLPLVEHGLQLAEQFKQKALGLLNFAGKQAQEATPVIQSNFAKLEQFWANDMWNFISGIQYLAGLSTLPGNPAIASLMSFTAAVTSPLTTQQTLLFNILGGWVASQIRDSDASTAFVVSGLAGAAVGSVGLGKILVDILPGYGAGVRGAVVTFKIMSGEMPSTEDLVNLLPAILSPGALVVEVVCPAILRRHVGPGEGAVQWMNRLIAFASRGNHVSPTHYVPESDAARRVTTILSSLTVTSLLRRLHKWINEDCSTPCAESWLWEVWDWVLHVLSDFKTCLKAKFVPLMPGIPLLSWPRGYKGEWRGDGVMHTTCPCGADLAGHIKNGSMRITGPKTCSNTWHGTFPINAYTTGPGVPIPAPNYKFALWRVSAEDYVEVRRVGDFHYVTGVTQDNIKFPCQVPAPELFTEVDGIRIHRHAPKCKPLLRDEVSFSVGLNSFVVGSQLPCEPEPDVAVLTSMLTDPSHITAESARRRLARGSRPSLASSSASQLSPRLLQATCTAPHDSPGTDLLEANLLWGSTATRVETDEKVIILDSFESCVAEQNDDREVSVAAEILRPTKKFPPALPIWARPDYNPPLTETWKQQDYQAPTVHGCALPPAKQPPVPSPRRKRTVQLTESVVSTALAELAAKTFGQSEPSSDRDTDLTTPTETTDSGPIVVDDASDDGSYSSMPPLEGEPGDPDLTSDSWSTVSGSEDVVCCSMSYSWTGALVTPCAAEESKLPISPLSNSLLRHHNMVYATTTRSAVTRQKKVTFDRLQVVDSTYNEVLKEIKARASRVKPRLLTTEEACDLTPPHSARSKFGYGKKDVRSHSRKAINHISSVWKDLLDDNNTPIPTTIMAKNEVFAVNPAKGGRKPARLIVYPDLGSRVCEKRALHDVIKKTALAVMGAAYGFQYSPAQRVEFLLTAWKSKNDPMGFSYDTRCFDSTVTEKDIRVEEEVYQCCDLEPEARKVITALTDRLYVGGPMHNSKGDLCGYRRCRATGVYTTSFGNTLTCYLKATAAIRAAALRDCTMLVCGDDLVVIAESDGVEEDNRALRAFTEAMTRYSAPPGDAPQPAYDLELITSCSSNVSVAHDVTGKKVYYLTRDPETPLARAVWETVRHTPVNSWLGNIIVYAPTIWVRMILMTHFFSILQSQEALEKALDFDMYGVTYSITPLDLPAIIQRLHGLSAFTLHGYSPHELNRVAGALRKLGVPPLRAWRHRARAVRAKLIAQGGRAKICGIYLFNWAVKTKLKLTPLPAAAKLDLSGWFTVGAGGGDIYHSMSHARPRYLLLCLLILTVGVGIFLLPAR
  
Inhibitor
Name:
BDBM35553
Synonyms:
1H-benzo[de]isoquinoline-1,3(2H)-dione, 1
Type:
Small organic molecule
Emp. Form.:
C20H15BrN2O3
Mol. Mass.:
411.249
SMILES:
OCCNc1ccc2C(=O)N(C(=O)c3cccc1c23)c1cccc(Br)c1
Structure:
Search PDB for entries with ligand similarity:
Substrate
Name:
poly(A)/oligo(U)18
Synonyms:
n/a
Type:
homopolymeric template/primer
Mol. Mass.:
358.43
Organism:
n/a
Description:
UTP/3H-UTP as co-substrate.
Residue:
3
Sequence:
NA