Reaction Details Report a problem with these data
Target
Rhomboid protease GluP [F193S,L370F,L379S,R499W]
Ligand
BDBM165194
Substrate
n/a
Meas. Tech.
Activity-based Protein Profiling (ABPP)
pH
7.4±0
Temperature
310.15±0 K
IC50
2.5e+4± 6e+3 nM
Citation
 Wolf, EV; Zeissler, A; Verhelst, SH Inhibitor Fingerprinting of Rhomboid Proteases by Activity-Based Protein Profiling Reveals Inhibitor Selectivity and Rhomboid Autoprocessing. ACS Chem Biol 10:2325-33 (2015) [PubMed]  Article 
Target
Name:
Rhomboid protease GluP [F193S,L370F,L379S,R499W]
Synonyms:
GLUP_BACSU | Rhomboid protease (BsYqgP) | gluP | yqgP
Type:
Protein
Mol. Mass.:
56433.35
Organism:
Bacillus subtilis (Firmicutes)
Description:
P54493[F193S,L370F,L379S,R499W]
Residue:
507
Sequence:
MFLLEYTYWKIAAHLVNSGYGVIQAGESDEIWLEAPDKSSHDLVRLYKHDLDFRQEMVRDIEEQAERVERVRHQLGRRRMKLLNVFFSTEAPVDDWEEIAKKTFEKGTVSVEPAIVRGTMLRDDLQAVFPSFRTEDCSEEHASFENAQMARERFLSLVLKQEEQRKTEAAVFQNGKPTFTYLFIALQILMFSLLEINGGSTNTETLVAFGAKENSLIAQGEWWRLLTPIVLHIGIAHLAFNTLALWSVGTAVERMYGSGRFLLIYLAAGITGSIASFVFSPYPSAGASGAIFGCLGALLYVALSNRKMFLRTIGTNIIVIIIINLGFGFAVSNIDNSGHIGGLIGGFFAAAALGLPKAGAFGKRLLSAVFLIALAVGFSYYGLHSPSHQESALIQQASELYQEGKYEEVTELLNGEAAQKDASADLLKILAVSDIQIGEYDQAVSLLERAVKKEPKDHASYYNLALLYAEKNELAQAEKAIQTAVKLKPKEQRYKELQWQIENNKES
  
Inhibitor
Name:
BDBM165194
Synonyms:
4‐Chloro‐7‐nitro‐3‐[(5‐phenylpentyl)oxy]isochromen‐1‐one (21)
Type:
Small organic molecule
Emp. Form.:
C20H18ClNO5
Mol. Mass.:
387.814
SMILES:
[O-][N+](=O)c1ccc2c(Cl)c(OCCCCCc3ccccc3)oc(=O)c2c1
Structure:
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