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Target
Cyclin-dependent kinase 1/G2/mitotic-specific cyclin-B
Ligand
BDBM5558
Substrate
Histone H1
Meas. Tech.
Kinase Inhibition Assay
IC50
300±n/a nM
Citation
 Hardcastle, IR; Arris, CE; Bentley, J; Boyle, FT; Chen, Y; Curtin, NJ; Endicott, JA; Gibson, AE; Golding, BT; Griffin, RJ; Jewsbury, P; Menyerol, J; Mesguiche, V; Newell, DR; Noble, ME; Pratt, DJ; Wang, LZ; Whitfield, HJ N2-substituted O6-cyclohexylmethylguanine derivatives: potent inhibitors of cyclin-dependent kinases 1 and 2. J Med Chem 47:3710-22 (2004) [PubMed]  Article 
Target
Name:
Cyclin-dependent kinase 1/G2/mitotic-specific cyclin-B
Synonyms:
CDK1/Cyclin B | Cyclin-Dependent Kinase 1 (CDK1) | p34cdc2/Cyclin B
Type:
Protein Complex
Mol. Mass.:
n/a
Description:
p34cdc2/cyclin B was purified from M-phase starfish oocytes by affinity chromatography.
Components:
This complex has 2 components.
Component 1
Name:
Cyclin-dependent kinase 1
Synonyms:
CDK1_ORYLA | Cell division control protein 2 (cdc2) | P34 protein kinase | cdc2 | cdk1
Type:
Enzyme Subunit
Mol. Mass.:
34693.35
Organism:
Marthasterias glacialis (starfish)
Description:
Q9DGD3 {empty hard to find)
Residue:
303
Sequence:
MEDYVKIEKIGEGTYGVVYKGRHKSTGQVVAMKKIRLESEEEGVPSTAVREVSLLQELKHPNVVRLLDVLMQESRLYLIFEFLSMDLKKYLDSIPSGQYMDPMLVKSYLYQILEGIYFCHRRRVLHRDLKPQNLLIDNKGVIKLADFGLARAFGVPVRVYTHEVVTLWYRAPEVLLGSPRYSTPVDVWSTGTIFAELATKKPLFHGDSEIDQLFRIFRTLGTPNNDVWPDVESLPDYKNTFPKWKEGSLSSMVKNLDKNGLDLLAKMLIYNPPKRISAREAMTHPYFDDLDKSTLPAACINGV
  
Component 2
Name:
G2/mitotic-specific cyclin-B
Synonyms:
CCNB_MARGL | Cyclin B | G2/mitotic-specific cyclin-B1
Type:
Enzyme Subunit
Mol. Mass.:
43790.28
Organism:
Marthasterias glacialis (starfish)
Description:
n/a
Residue:
388
Sequence:
MLNGENVDSRIMGKVATRASSKGVKSTLGTRGALENISNVARNNLQAGAKKELVKAKRGMTKSKATSSLQSVMGLNVEPMEKAKPQSPEPMDMSEINSALEAFSQNLLEGVEDIDKNDFDNPQLCSEFVNDIYQYMRKLEREFKVRTDYMTIQEITERMRSILIDWLVQVHLRFHLLQETLFLTIQILDRYLEVQPVSKNKLQLVGVTSMLIAAKYEEMYPPEIGDFVYITDNAYTKAQIRSMECNILRRLDFSLGKPLCIHFLRRNSKAGGVDGQKHTMAKYLMELTLPEYAFVPYDPSEIAAAALCLSSKILEPDMEWGTTLVHYSAYSEDHLMPIVQKMALVLKNAPTAKFQAVRKKYSSAKFMNVSTISALTSSTVMDLADQMC
  
Inhibitor
Name:
BDBM5558
Synonyms:
2-(4-{[6-(cyclohexylmethoxy)-9H-purin-2-yl]amino}phenyl)acetonitrile | 6-Cyclohexylmethoxy-2-(4 -cyanomethylanilino)purine | O6-Cyclohexylmethylguanine deriv. 42
Type:
Small organic molecule
Emp. Form.:
C20H22N6O
Mol. Mass.:
362.4283
SMILES:
N#CCc1ccc(Nc2nc(OCC3CCCCC3)c3[nH]cnc3n2)cc1
Structure:
Search PDB for entries with ligand similarity:
Substrate
Name:
Histone H1
Synonyms:
Sigma Type IIIS
Type:
Other Protein Type
Mol. Mass.:
358.43
Organism:
Bos taurus (bovine)
Description:
12.5 uM ATP as co-substrate.
Residue:
3
Sequence:
NA