Reaction Details Report a problem with these data
Target
cAMP-dependent protein kinase type I-alpha regulatory subunit [K244A]
Ligand
BDBM218808
Substrate
n/a
Meas. Tech.
8-[Fluo]-cAMP Fluorescence Polarization Binding Assay
Kd
7.71±0.11 nM
Citation
 P Barros, E; Malmstrom, RD; Nourbakhsh, K; Del Rio, JC; Kornev, AP; Taylor, SS; Amaro, RE Electrostatic Interactions as Mediators in the Allosteric Activation of Protein Kinase A RIa. Biochemistry 56:1536-1545 (2017) [PubMed]  Article 
Target
Name:
cAMP-dependent protein kinase type I-alpha regulatory subunit [K244A]
Synonyms:
KAP0_HUMAN | PKR1 | PRKAR1 | PRKAR1A | Protein kinase A Iα regulatory subunit (RIα K242A) | TSE1
Type:
Protein
Mol. Mass.:
42916.02
Organism:
Homo sapiens (Human)
Description:
Human RIα B/C Helix K242A mutant
Residue:
381
Sequence:
MESGSTAASEEARSLRECELYVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEKEEAKQIQNLQKAGTRTDSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFSVSFIAGETVIQQGDEGDNFYVIDQGETDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLRKRAMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQGEPGDEFFIILEGSAAVLQRRSENEEFVEVGRLGPSDYFGEIALLMNRPRAATVVARGPLKCVKLDRPRFERVLGPCSDILKRNIQQYNSFVSLSV
  
Inhibitor
Name:
BDBM218808
Synonyms:
8-[Fluo]-cAMP
Type:
Small organic molecule
Emp. Form.:
C33H27N7O11PS2
Mol. Mass.:
792.712
SMILES:
Nc1ncnc2n(C3OC4COP([O-])(=O)OC4C3O)c(SCCNC(=S)Nc3ccc(c(c3)C(O)=O)-c3c4ccc(O)cc4oc4cc(=O)ccc34)nc12 |(-9.02,-3.05,;-9.02,-4.59,;-10.36,-5.36,;-10.36,-6.9,;-9.02,-7.67,;-7.69,-6.9,;-6.23,-7.38,;-6.23,-8.92,;-7.56,-8.15,;-8.89,-8.92,;-10.38,-8.52,;-11.47,-9.61,;-12.96,-10.01,;-13.73,-8.67,;-13.73,-11.34,;-11.47,-10.41,;-10.38,-9.32,;-7.32,-10.01,;-7.32,-11.55,;-5.32,-6.13,;-3.78,-6.13,;-3.01,-4.8,;-2.24,-6.13,;-.91,-5.36,;.43,-4.59,;.43,-3.05,;1.76,-5.36,;3.3,-5.36,;4.07,-4.03,;5.61,-4.03,;6.38,-5.36,;5.61,-6.7,;4.07,-6.7,;6.01,-8.18,;4.92,-9.27,;7.5,-8.58,;7.92,-5.36,;8.69,-6.7,;7.92,-8.03,;8.69,-9.36,;10.23,-9.36,;11,-10.7,;11,-8.03,;10.23,-6.7,;11,-5.36,;10.23,-4.03,;11,-2.7,;10.23,-1.36,;11,-.03,;8.69,-1.36,;7.92,-2.69,;8.69,-4.03,;-6.23,-4.89,;-7.69,-5.36,)|
Structure:
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