Reaction Details Report a problem with these data
Target
Phosphoenolpyruvate carboxykinase, cytosolic [GTP] [H477R]
Ligand
BDBM223202
Substrate
n/a
Meas. Tech.
PEPCK Inhibition Assay
Ki
6.96e+5± 2.18e+5 nM
Citation
 Cui, DS; Broom, A; Mcleod, MJ; Meiering, EM; Holyoak, T Asymmetric Anchoring Is Required for Efficient O-Loop Opening and Closing in Cytosolic Phosphoenolpyruvate Carboxykinase. Biochemistry 56:2106-2115 (2017) [PubMed]  Article 
Target
Name:
Phosphoenolpyruvate carboxykinase, cytosolic [GTP] [H477R]
Synonyms:
PCKGC_RAT | Pck1 | Phosphoenolpyruvate carboxykinase (PEPCK H477R)
Type:
Enzyme
Mol. Mass.:
69434.03
Organism:
Rattus norvegicus (Rat)
Description:
Rat cytosolic PEPCK H477R mutant
Residue:
622
Sequence:
MPPQLHNGLDFSAKVIQGSLDSLPQEVRKFVEGNAQLCQPEYIHICDGSEEEYGRLLAHMQEEGVIRKLKKYDNCWLALTDPRDVARIESKTVIITQEQRDTVPIPKSGQSQLGRWMSEEDFEKAFNARFPGCMKGRTMYVIPFSMGPLGSPLAKIGIELTDSPYVVASMRIMTRMGTSVLEALGDGEFIKCLHSVGCPLPLKKPLVNNWACNPELTLIAHLPDRREIISFGSGYGGNSLLGKKCFALRIASRLAKEEGWLAEHMLILGITNPEGKKKYLAAAFPSACGKTNLAMMNPTLPGWKVECVGDDIAWMKFDAQGNLRAINPENGFFGVAPGTSVKTNPNAIKTIQKNTIFTNVAETSDGGVYWEGIDEPLAPGVTITSWKNKEWRPQDEEPCAHPNSRFCTPASQCPIIDPAWESPEGVPIEGIIFGGRRPAGVPLVYEALSWQHGVFVGAAMRSEATAAAEHKGKVIMRDPFAMRPFFGYNFGKYLAHWLSMAHRPAAKLPKIFHVNWFRKDKNGKFLWPGFGENSRVLEWMFGRIEGEDSAKLTPIGYVPKEDALNLKGLGDVNVEELFGISKEFWEKEVEEIDKYLEDQVNADLPYEIERELRALKQRISQM
  
Inhibitor
Name:
BDBM223202
Synonyms:
β,γ-methyleneguanosine 5′-triphosphate | GMPPCP
Type:
Small organic molecule
Emp. Form.:
C11H17N5O13P3
Mol. Mass.:
520.2002
SMILES:
Nc1nc([O-])c2ncn([C@@H]3O[C@H](COP(O)(=O)OP(O)(=O)C[P+](O)(O)[O-])[C@@H](O)[C@H]3O)c2n1
Structure:
Search PDB for entries with ligand similarity: