Ki Summary BindingDB logo
myBDB logout
Reaction Details
Report a problem with these data
TargetBromodomain protein 4 bromodomain 1 (BRD4 BD1 M149A)
LigandBDBM227644
Substrate/Competitorn/a
Meas. Tech.TR-FRET Competition Assay
pH7.5±0
Temperature277.15±0 K
Kd 7.27e+4± 1.0e+4 nM
Citation Jung, MPhilpott, MMüller, SSchulze, JBadock, VEberspächer, UMoosmayer, DBader, BSchmees, NFernández-Montalván, AHaendler, B Affinity map of bromodomain protein 4 (BRD4) interactions with the histone H4 tail and the small molecule inhibitor JQ1. J Biol Chem289:9304-19 (2014) [PubMed]  Article
More Info.:Get all data from this article,  Assay Method
 
Bromodomain protein 4 bromodomain 1 (BRD4 BD1 M149A)
Name:Bromodomain protein 4 bromodomain 1 (BRD4 BD1 M149A)
Synonyms:n/a
Type:Protein
Mol. Mass.:14550.16
Organism:Homo sapiens (Human)
Description:Human BRD4 BD1 (44-166 aa) M149A mutant
Residue:123
Sequence:
NPPPPETSNPNKPKRQTNQLQYLLRVVLKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKT
PMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLAAEALEKLFLQKINE
LPT
Blast this sequence in BindingDB or PDB
  Blast E-value cutoff:
BDBM227644
NameBDBM227644
Synonyms:Histone H4 (1-25) | MSGRGKGGKGLGKGGAKRHRKVLRD
TypeSmall organic molecule
Emp. Form.C108H196N44O29S
Mol. Mass.2607.054
SMILESCSCC[C@H](N)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CCCNC(N)=N)C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)NCC(=O)NCC(=O)N[C@@H](CCCCN)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)NCC(=O)NCC(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](Cc1cnc[nH]1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(O)=O |r|
Structure
Search PDB for entries with ligand similarity:Similarity to this molecule at least:
n/a