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Target
Cyclin-dependent kinase 1/G2/mitotic-specific cyclin-B
Ligand
BDBM7405
Substrate
Histone H1
Meas. Tech.
Kinase Inhibition Assay
pH
7.2±n/a
Temperature
303.15±n/a K
IC50
92000±n/a nM
Citation
 Polychronopoulos, P; Magiatis, P; Skaltsounis, AL; Myrianthopoulos, V; Mikros, E; Tarricone, A; Musacchio, A; Roe, SM; Pearl, L; Leost, M; Greengard, P; Meijer, L Structural basis for the synthesis of indirubins as potent and selective inhibitors of glycogen synthase kinase-3 and cyclin-dependent kinases. J Med Chem 47:935-46 (2004) [PubMed]  Article 
Target
Name:
Cyclin-dependent kinase 1/G2/mitotic-specific cyclin-B
Synonyms:
CDK1/Cyclin B | Cyclin-Dependent Kinase 1 (CDK1) | p34cdc2/Cyclin B
Type:
Protein Complex
Mol. Mass.:
n/a
Description:
p34cdc2/cyclin B was purified from M-phase starfish oocytes by affinity chromatography.
Components:
This complex has 2 components.
Component 1
Name:
Cyclin-dependent kinase 1
Synonyms:
CDK1_ORYLA | Cell division control protein 2 (cdc2) | P34 protein kinase | cdc2 | cdk1
Type:
Enzyme Subunit
Mol. Mass.:
34693.35
Organism:
Marthasterias glacialis (starfish)
Description:
Q9DGD3 {empty hard to find)
Residue:
303
Sequence:
MEDYVKIEKIGEGTYGVVYKGRHKSTGQVVAMKKIRLESEEEGVPSTAVREVSLLQELKHPNVVRLLDVLMQESRLYLIFEFLSMDLKKYLDSIPSGQYMDPMLVKSYLYQILEGIYFCHRRRVLHRDLKPQNLLIDNKGVIKLADFGLARAFGVPVRVYTHEVVTLWYRAPEVLLGSPRYSTPVDVWSTGTIFAELATKKPLFHGDSEIDQLFRIFRTLGTPNNDVWPDVESLPDYKNTFPKWKEGSLSSMVKNLDKNGLDLLAKMLIYNPPKRISAREAMTHPYFDDLDKSTLPAACINGV
  
Component 2
Name:
G2/mitotic-specific cyclin-B
Synonyms:
CCNB_MARGL | Cyclin B | G2/mitotic-specific cyclin-B1
Type:
Enzyme Subunit
Mol. Mass.:
43790.28
Organism:
Marthasterias glacialis (starfish)
Description:
n/a
Residue:
388
Sequence:
MLNGENVDSRIMGKVATRASSKGVKSTLGTRGALENISNVARNNLQAGAKKELVKAKRGMTKSKATSSLQSVMGLNVEPMEKAKPQSPEPMDMSEINSALEAFSQNLLEGVEDIDKNDFDNPQLCSEFVNDIYQYMRKLEREFKVRTDYMTIQEITERMRSILIDWLVQVHLRFHLLQETLFLTIQILDRYLEVQPVSKNKLQLVGVTSMLIAAKYEEMYPPEIGDFVYITDNAYTKAQIRSMECNILRRLDFSLGKPLCIHFLRRNSKAGGVDGQKHTMAKYLMELTLPEYAFVPYDPSEIAAAALCLSSKILEPDMEWGTTLVHYSAYSEDHLMPIVQKMALVLKNAPTAKFQAVRKKYSSAKFMNVSTISALTSSTVMDLADQMC
  
Inhibitor
Name:
BDBM7405
Synonyms:
(2 Z,3 E)-6-Bromo-1-methylindirubin-3 -oxime | 6-bromo-3-[(2Z,3E)-3-(hydroxyimino)-2,3-dihydro-1H-indol-2-ylidene]-1-methyl-2,3-dihydro-1H-indol-2-one | 6-bromo-N-methyl-indirubin-3 -oxime | indirubin deriv. 13c
Type:
Small organic molecule
Emp. Form.:
C17H12BrN3O2
Mol. Mass.:
370.2
SMILES:
CN1C(=O)C(c2ccc(Br)cc12)c1[nH]c2ccccc2c1N=O
Structure:
Search PDB for entries with ligand similarity:
Substrate
Name:
Histone H1
Synonyms:
n/a
Type:
Other Protein Type
Mol. Mass.:
358.43
Organism:
Bos taurus (bovine)
Description:
n/a
Residue:
3
Sequence:
NA