Reaction Details Report a problem with these data
Target
Glycogen synthase kinase-3 beta
Ligand
BDBM7427
Substrate
GSK-3 Peptide Substrate
Meas. Tech.
Kinase Inhibition Assay
Temperature
303.15±n/a K
IC50
7±n/a nM
Comments
extracted
Citation
 Polychronopoulos, P; Magiatis, P; Skaltsounis, AL; Myrianthopoulos, V; Mikros, E; Tarricone, A; Musacchio, A; Roe, SM; Pearl, L; Leost, M; Greengard, P; Meijer, L Structural basis for the synthesis of indirubins as potent and selective inhibitors of glycogen synthase kinase-3 and cyclin-dependent kinases. J Med Chem 47:935-46 (2004) [PubMed]  Article 
Target
Name:
Glycogen synthase kinase-3 beta
Synonyms:
GSK-3 alpha/beta | Glycogen Synthase Kinase-3, beta
Type:
Enzyme Mixture
Mol. Mass.:
48062.20
Organism:
Porcine
Description:
Purification of GSK-3 alpha and GSK-3 beta from Porcine Brain on Axin-Sepharose. Porcine brains were obtained from a local slaughter and directly homogenized and processed for affinity chromatography.
Residue:
433
Sequence:
MSGRPRTTSFAESCKPVQQPSAFGSMKVSRDKDGSKVTTVVATPGQGPDRPQEVSYTDTKVIGNGSFGVVYQAKLCDSGELVAIKKVLQDKRFKNRELQIMRKLDHCNIVRLRYFFYSSGEKKDEVYLNLVLDYVPETVYRVARHYSRAKQTLPVIYVKLYMYQLFRSLAYIHSFGICHRDIKPQNLLLDPDTAVLKLCDFGSAKQLVRGEPNVSYICSRYYRAPELIFGATDYTSSIDVWSAGCVLAELLLGQPIFPGDSGVDQLVEIIKVLGTPTREQIREMNPNYTEFKFPQIKAHPWTKDSSGTGHFTSGVRVFRPRTPPEAIALCSRLLEYTPTARLTPLEACAHSFFDELRDPNVKLPNGRDTPALFNFTTQELSSNPPLATILIPPHARIQAAASTPSNATAASDTNAGDRGQTNNTASASASNST
  
Inhibitor
Name:
BDBM7427
Synonyms:
(2 Z,3 E)-6-Bromo-5-nitroindirubin-3 -oxime | 6-Bromo-5-nitroindirubin-3 -oxime | 6-bromo-3-[(2Z,3E)-3-(hydroxyimino)-2,3-dihydro-1H-indol-2-ylidene]-5-nitro-2,3-dihydro-1H-indol-2-one | indirubin deriv. 7g
Type:
Small organic molecule
Emp. Form.:
C16H9BrN4O4
Mol. Mass.:
401.171
SMILES:
[O-][N+](=O)c1cc2[c-](-c3[nH]c4ccccc4c3N=[OH+])c(=O)[nH]c2cc1Br |(.96,8.59,;2.41,8.07,;3.5,9.15,;2.56,6.53,;1.3,5.64,;1.44,4.11,;.4,2.98,;-1.14,2.98,;-2.04,1.73,;-3.51,2.2,;-4.84,1.43,;-6.18,2.2,;-6.18,3.75,;-4.84,4.52,;-3.51,3.75,;-2.04,4.22,;-1.57,5.69,;-2.66,6.77,;1.16,1.63,;.51,.23,;2.67,1.94,;2.84,3.47,;4.1,4.36,;3.96,5.89,;5.21,6.78,)|
Structure:
Search PDB for entries with ligand similarity:
Substrate
Name:
GSK-3 Peptide Substrate
Synonyms:
GS-1 Peptide | Phospho-GS2 Substrate Peptide
Type:
Peptide
Mol. Mass.:
3045.17
Organism:
n/a
Description:
n/a
Residue:
27
Sequence:
YRRAAVPPSPSLSRHSSPHQSPEDEEE