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Assay Method Information

Assay Name:  MAO Inhibition Assay
Description:  MAO A and MAO B activities were determined spectrophotometrically. Competitive Ki values for both enzymes were determined by measuring initial rates of substrate oxidation in the presence of varying concentrations of inhibitor. Apparent Km values for each inhibitor concentration (slopes of double reciprocal plots) were plotted as a function of inhibitor concentration, and the Ki values were determined.
Affinity data for this assay
 

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Last update November 1, 2007
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