| Assay Method Information | |
| | Luciferase reporter assay |
| Description: | 10 μL of supernatant from the assay was transferred to white 384-plate with flat bottom and squared wells. One pouch of QUANTI-Luc Plus was dissolved in 25 mL of water. 100 μL of QLC Stabilizer per 25 mL of QUANTI-Luc Plus solution was added. 50 μL of QUANTI-Luc Plus/QLC solution per well was then added. Luminescence was measured on a Platereader (e.g., Spectramax I3X (Molecular Devices GF3637001)). |
| Affinity data for this assay | |
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