Assay Method Information

Assay Name:  CDK Inhibition Assay
Description:  In vitro kinase assay using purified CDK2/Cyclin A was incubated at room temperature with substrate, and test compounds in the presence of 100 uM ATP/ [gamma-33P] ATP. Assays were performed in 96-well polypropylene plates. 33P incorporation into substrate was measured using a scintillation counter.
Affinity data for this assay
 

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