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Assay Method Information

Assay Name:  PKB In Vitro Kinase Assay
Description:  An ELISA-based assay has been developed for the serine/threonine kinase, PKB-alpha. The assay utilizes an N-terminally biotinylated substrate peptide. When this substrate is phosphorylated by the kinase, the product is recognized by a substrate/sequence-specific antibody, which binds to particular phosphorylated serine residues. The amount of phosphorylated peptide was measured by the enzyme-catalyzed ABTS conversion and photometrical measurement at 405 nm. Calculation of IC50 values was performed with ActivityBase.
Affinity data for this assay
 

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Last update November 1, 2007
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