BindingDB logo
myBDB logout

Assay Method Information

Assay Name:  Ligand Binding Assay
Description:  A fluorescence polarization anisotropy (FPA) competition assay was used to determine the affinities of compounds to BIR3 protein. Fluorescence polarization measurements were carried out on an Analyst 96-well plate reader. 6-Carboxyfluorescein (FAM) labeled peptides with the sequences AVPIAQK (FAM)-NH2 was used as a probe. Dissociation constants were determined from titration curves with in-house written software.
Affinity data for this assay
 

If you find an error in this entry please send us an E-mail
   
    

Home

|

Search

|

Deposit

|

SiteMap

|

About us

|

Email us

|

Info

 
Last update November 1, 2007
©2000 BindingDB. All rights reserved.